Macdonald N P, Menachery A, Reboud J, Cooper J M
ARC Centre of Excellence for Electromaterials Science (ACES), Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Faculty of Science, Engineering and Technology, University of Tasmania Private Bag 75 Hobart TAS 7001 Australia.
Division of Biomedical Engineering, School of Engineering, University of Glasgow Rankine Building, Oakfield Avenue Glasgow G12 8LT UK
RSC Adv. 2018 Mar 6;8(18):9603-9610. doi: 10.1039/c8ra00849c. eCollection 2018 Mar 5.
We report upon a novel coplanar dielectrophoresis (DEP) based cell patterning system for generating transferrable hepatic cell constructs, resembling a liver-lobule, in culture. The use of paper reinforced gel substrates provided sufficient strength to enable these constructs to be transfered into 96-well plates for long term functional studies, including in the future, drug development studies. Experimental results showed that hepatic cells formed DEP field-induced structures corresponding to an array of lobule-mimetic patterns. Hepatic viability was observed over a period of 3 days by the use of a fluorescent cell staining technique, whilst the liver specific functionality of albumin secretion showed a significant enhancement due to the layer patterning of cell lines (HepG2/C3A), compared to 2D patterned cells and un-patterned control. This "build and transfer" concept could, in future, also be adapted for the layer-by-layer construction of organs-on-chip in microtitre formats.
我们报道了一种基于新型共面介电泳（DEP）的细胞图案化系统，用于在培养中生成可转移的类似肝小叶的肝细胞构建体。使用纸增强凝胶底物提供了足够的强度，使这些构建体能够转移到96孔板中进行长期功能研究，包括未来的药物开发研究。实验结果表明，肝细胞形成了与一系列小叶模拟图案相对应的DEP场诱导结构。通过使用荧光细胞染色技术，在3天的时间内观察到肝细胞活力，同时与二维图案化细胞和未图案化对照相比，由于细胞系（HepG2/C3A）的层图案化，白蛋白分泌的肝脏特异性功能显著增强。这种“构建并转移”的概念未来也可适用于微量滴定格式的芯片器官逐层构建。
