Zhang Jingjing, Guo Feifei, Wu Hongwei, Wei Junying, Xian Minghua, Fan Fangfang, Tang Shihuan, Zhao Ye, Zhang Yi, Li Defeng, Yang Hongjun
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700 China
College of Ethnic Medicine, Chengdu University of TCM Chengdu 610072 China.
RSC Adv. 2018 Mar 12;8(18):10032-10039. doi: 10.1039/c7ra13326j. eCollection 2018 Mar 5.
Yixin-Shu capsules (YXS) are a Chinese Materia Medica standardized product used for heart disease and their effectiveness has been demonstrated through both clinical and experimental research. However, the mechanism involved has remained unclear. The effect of YXS on the cardiac-like differentiation of mesenchymal stem cells was investigated in this study. The intestinal absorption liquid of YXS was prepared using an intestinal absorption method and 62 compounds have been identified. A compound-target-function network constructed by a network pharmacology-based approach indicated that these compounds had an effect on cell differentiation. The effects of YXS on cardiac-like differentiation of mesenchymal stem cells was verified by detecting cardiac-specific protein expression such as α-actinin, cardiac troponin-I and desmin through real time-PCR, western blotting and immunofluorescence staining. A network pharmacology analysis indicated that the facilitation of YXS on the cardiac-like differentiation may be through the TGF-β signaling pathway, Wnt signaling pathway and MAPK signaling pathway. The observed improvements on cardiac differentiation may be due to the novel molecular mechanism for YXS that could also benefit developments in cardiac tissue engineering.
益心舒胶囊（YXS）是一种用于治疗心脏病的中药标准化产品，其有效性已通过临床和实验研究得到证实。然而，其作用机制尚不清楚。本研究探讨了YXS对间充质干细胞向心肌样细胞分化的影响。采用肠道吸收法制备了YXS的肠道吸收液，并鉴定出62种化合物。通过基于网络药理学的方法构建的化合物-靶点-功能网络表明，这些化合物对细胞分化有影响。通过实时定量聚合酶链反应、蛋白质免疫印迹和免疫荧光染色检测心肌特异性蛋白如α-辅肌动蛋白、心肌肌钙蛋白-I和结蛋白的表达，验证了YXS对间充质干细胞向心肌样细胞分化的影响。网络药理学分析表明，YXS促进心肌样细胞分化可能是通过转化生长因子-β信号通路、Wnt信号通路和丝裂原活化蛋白激酶信号通路。观察到的心肌分化改善可能归因于YXS的新分子机制，这也可能有益于心脏组织工程的发展。
