一种基于生物质量子点与有机染料之间的共振能量转移用于检测二氧化硫衍生物的新型比率荧光测定法。

A new ratiometric fluorescence assay based on resonance energy transfer between biomass quantum dots and organic dye for the detection of sulfur dioxide derivatives.

作者信息

Zhao Jingjin, Peng Yao, Yang Keqin, Chen Yunyun, Zhao Shulin, Liu Yi-Ming

机构信息

State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University Guilin 541004 China

Department of Chemistry and Biochemistry, Jackson State University 1400 Lynch St. Jackson MS 39217 USA

出版信息

RSC Adv. 2019 Dec 17;9(71):41955-41961. doi: 10.1039/c9ra09437g. eCollection 2019 Dec 13.

Sulfur dioxide (SO) is considered as the fourth gas signal molecule after nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (HS). It plays important roles in several physiological processes. Therefore, the design and synthesis of nanoprobes for the detection of SO derivatives in cells is of great significance. Herein, we report a new ratiometric fluorescence nanoprobe based on resonance energy transfer (RET) between biomass quantum dots (BQDs) and organic dye (DMI) for the detection of SO derivatives. The proposed ratiometric fluorescence assay allows the determination of HSO in the range of 1.0 to 225 μM with a detection limit of 0.5 μM. Importantly, the proposed ratiometric fluorescence nanoprobe exhibits a high photostability and good selectivity for HSO over other chemical species including HS and biological mercaptans. Quantitation of HSO in cell lysates by using the nanoprobe is demonstrated.

二氧化硫（SO）被认为是继一氧化氮（NO）、一氧化碳（CO）和硫化氢（HS）之后的第四种气体信号分子。它在多个生理过程中发挥着重要作用。因此，设计和合成用于检测细胞中SO衍生物的纳米探针具有重要意义。在此，我们报道了一种基于生物质量子点（BQDs）与有机染料（DMI）之间的共振能量转移（RET）的新型比率荧光纳米探针，用于检测SO衍生物。所提出的比率荧光测定法能够在1.0至225μM范围内测定亚硫酸氢根（HSO₃⁻），检测限为0.5μM。重要的是，所提出的比率荧光纳米探针对亚硫酸氢根（HSO₃⁻）表现出高光稳定性，并且对包括硫化氢（HS）和生物硫醇在内的其他化学物质具有良好的选择性。利用该纳米探针测定细胞裂解液中亚硫酸氢根（HSO₃⁻）的含量得到了证实。