Lee Hyeonjeong, Hwang Jeongin, Park Yunsung, Kwon Donghoon, Lee Sanghee, Kang Inseok, Jeon Sangmin
Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH) 77 Cheongam-Ro, Nam-Gu Pohang Gyeongbuk Republic of Korea
RSC Adv. 2018 Jul 23;8(46):26266-26270. doi: 10.1039/c8ra04739a. eCollection 2018 Jul 19.
We developed a facile method for the detection of pathogenic bacteria using gold-coated magnetic nanoparticle clusters (Au@MNCs) and porous nitrocellulose strips. Au@MNCs were synthesized and functionalized with half-fragments of O157 antibodies. After the nanoparticles were used to capture O157 in milk and dispersed in a buffer solution, one end of a test strip was dipped into the solution. Due to the size difference between the -Au@MNC complexes (approximately 1 μm) and free Au@MNCs (approximately 180 nm), only -Au@MNC complexes accumulated at the meniscus of the test strip and induced a color change. The color intensity of the meniscus was proportional to the concentration, and the detection limit for in milk was 10 CFU mL by the naked eye. The presence of -Au@MNC complexes at the meniscus was confirmed using a real-time PCR assay. The developed method was highly selective for when compared with , , and .
我们开发了一种使用金包被磁性纳米颗粒簇（Au@MNCs）和多孔硝酸纤维素试纸条检测病原菌的简便方法。合成了Au@MNCs并用O157抗体的半片段进行功能化。纳米颗粒用于捕获牛奶中的O157并分散在缓冲溶液中后，将试纸条的一端浸入该溶液中。由于-Au@MNC复合物（约1μm）和游离Au@MNCs（约180nm）之间存在尺寸差异，只有-Au@MNC复合物在试纸条的弯月面处聚集并引起颜色变化。弯月面处的颜色强度与浓度成正比，牛奶中肉眼检测限为10 CFU/mL。使用实时PCR测定法确认了弯月面处存在-Au@MNC复合物。与、和相比，所开发的方法对具有高度选择性。
