Blanchard E, Nonell A, Chartier F, Rincel A, Bresson C
Den - Service d'Etudes Analytiques et de Réactivité des Surfaces (SEARS) - Laboratoire de développement Analytique Nucléaire, Isotopique et Elémentaire (LANIE), CEA, Université Paris-Saclay F-91191 Gif-sur-Yvette France
Sorbonne Universités F-75005 Paris France.
RSC Adv. 2018 Jul 9;8(44):24760-24772. doi: 10.1039/c8ra02961j.
In this work, amide-bonded columns packed with fully porous particles (FPP) and superficially porous particles (SPP) were evaluated to separate lanthanide-polyaminocarboxylic species by hydrophilic interaction liquid chromatography (HILIC), using two model samples of interest in nuclear and other industrial applications. We assessed the gains achieved by reducing the dimensions of the columns along with the size of the FPPs to sub-2 μm and by using sub-3 μm SPP-packed columns. The FPP-packed Acquity column (100 × 2.1 mm; 1.7 μm) performed better than the SPP-packed Accucore column (150 × 2.1 mm; 2.6 μm), with a separation that was two times more efficient and three times shorter, while generating around 30% less in effluent volumes. This column was also coupled simultaneously to electrospray ionisation mass spectrometry (ESIMS) and inductively coupled plasma mass spectrometry (ICPMS). The instrumental set-up was performed in a conventional laboratory, by taking into account the geometrical constraints existing in the laboratory dedicated to radioelement analysis. Furthermore, separation of the series of lanthanide (Ln) species was demonstrated for the first time thanks to the separation mode of hydrophilic interaction liquid chromatography.
在本研究中，我们评估了填充全多孔颗粒（FPP）和表面多孔颗粒（SPP）的酰胺键合柱，通过亲水作用液相色谱（HILIC）分离镧系元素 - 聚氨基羧酸物种，使用了核工业及其他工业应用中感兴趣的两个模型样品。我们评估了通过将柱尺寸以及FPP尺寸减小至亚2μm，以及使用亚3μm SPP填充柱所取得的成效。填充FPP的Acquity柱（100×2.1 mm；1.7μm）比填充SPP的Accucore柱（150×2.1 mm；2.6μm）表现更好，分离效率提高了两倍，分离时间缩短了三倍，同时流出体积减少了约30%。该柱还同时与电喷雾电离质谱（ESIMS）和电感耦合等离子体质谱（ICPMS）联用。仪器设置在常规实验室中进行，考虑到专门用于放射性元素分析的实验室中存在的几何限制。此外，由于亲水作用液相色谱的分离模式，首次证明了一系列镧系（Ln）物种的分离。
