Feng Xiao-Chi, Guo Wan-Qian, Zheng He-Shan, Wu Qing-Lian, Luo Hai-Chao, Ren Nan-Qi
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology 73 Huanghe Road Harbin Heilongjiang 150090 P. R. China
RSC Adv. 2018 May 1;8(29):16178-16186. doi: 10.1039/c8ra02315h. eCollection 2018 Apr 27.
In order to understand the inhibitory mechanism of metabolic uncoupler in biofilm, this study investigated the effect of TCS on biofilm formation, flocculability, surface characteristics and thermodynamic properties. An optimal concentration of TCS, a metabolic uncoupler, was observed to substantially inhibit biofilm formation and the secretion of extracellular polymeric substances (EPS). The effect of TCS on the zeta potential and flocculability of bacterial suspension implied the addition of 100 μg L TCS increased the net negative charge of cell surface which induced the reduction of flocculability. Meanwhile, the effects of TCS on bacterial surfacial thermodynamic properties were analyzed by the Derjaguin-Landau-Verwey-Overbeek (DLVO) and extend DLVO (XDLVO) theories. As DLVO and XDLVO predicted, the primary energy barrier between bacterial cells incubated with 100 μg L TCS were increased compared to that of control, indicating that incubated with 100 μg L TCS must consume more energy to aggregate or form biofilm.
为了解代谢解偶联剂对生物膜的抑制机制，本研究考察了TCS对生物膜形成、絮凝性、表面特性和热力学性质的影响。观察到代谢解偶联剂TCS的最佳浓度可显著抑制生物膜形成和细胞外聚合物（EPS）的分泌。TCS对细菌悬浮液的ζ电位和絮凝性的影响表明，添加100 μg/L TCS会增加细胞表面的净负电荷，从而导致絮凝性降低。同时，采用Derjaguin-Landau-Verwey-Overbeek（DLVO）理论和扩展DLVO（XDLVO）理论分析了TCS对细菌表面热力学性质的影响。正如DLVO和XDLVO所预测的，与对照相比，用100 μg/L TCS处理的细菌细胞之间的主要能垒增加，这表明用100 μg/L TCS处理的细菌细胞聚集或形成生物膜时必须消耗更多能量。
