State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, 210046, China.
Appl Microbiol Biotechnol. 2012 Sep;95(5):1313-21. doi: 10.1007/s00253-011-3765-6. Epub 2011 Dec 13.
3,3',4',5-Tetrachlorosalicylanilide (TCS) is an effective metabolic uncoupler utilized for microbial yield reduction. However, its potential impact, in particular on the soluble microbial products (SMP) formation, is unknown yet. Herein we study the effect of TCS on SMP production and analyze the related mechanism. The addition of TCS in activated sludge system led to an increased production of SMP, especially proteins. The SMP were produced in proportion to the substrate utilization at a low TCS concentration, while more non-substrate-associated SMP were released at a high TCS concentration. TCS simulated the production of extracellular polymeric substances (EPS) and enhanced cell lysis, which both contributed to SMP production. FTIR and EEM analyses show that the SMP, EPS, and cell lysis products have similar functional groups and fluorescence properties, indicating a similar origin of these substances. In addition, a dose of TCS increased the release of high molecular weight compounds due to cell lysis. This study might benefit for a better understanding of the response of activated sludge to metabolic uncouplers like TCS.
3,3',4',5-四氯邻苯二甲酰亚胺（TCS）是一种有效的代谢解偶联剂，用于减少微生物产量。然而，其潜在影响，特别是对可溶性微生物产物（SMP）形成的影响，目前尚不清楚。本研究探讨了 TCS 对 SMP 产生的影响，并分析了相关机制。在活性污泥系统中添加 TCS 会导致 SMP，特别是蛋白质的产生增加。在低 TCS 浓度下，SMP 的产生与底物利用成正比，而在高 TCS 浓度下，会释放出更多与非底物相关的 SMP。TCS 模拟了细胞外聚合物（EPS）的产生并增强了细胞裂解，这两者都有助于 SMP 的产生。FTIR 和 EEM 分析表明，SMP、EPS 和细胞裂解产物具有相似的官能团和荧光特性，表明这些物质具有相似的来源。此外，TCS 的剂量增加了由于细胞裂解而释放的高分子化合物的释放。本研究有助于更好地理解活性污泥对 TCS 等代谢解偶联剂的反应。
