Chen Shunjie, Shan Jianping, Niu Wei, Lin Fujun, Liu Shuang, Wu Ping, Sun Lijing, Lu Wei, Jiang Gengru
Department of Nephrology, Xin Hua Hospital Affiliated to Shanghai JiaoTong University School of Medicine Shanghai 200092 P. R. China
RSC Adv. 2018 Apr 30;8(29):15890-15896. doi: 10.1039/c7ra13440a. eCollection 2018 Apr 27.
In this study, we have prepared miR-155 inhibitor-loaded liposome vesicles for the effective treatment of acute kidney injury. The efficacy of liposomal miR-155 inhibitor in the expression of miR-155, mortality in animals, the expression of TNF-α-IL6, and the expression of SOCS1-STAT1 were evaluated. The loading of miR-155 inhibitor into liposomes conferred the much needed colloidal stability and efficient delivery to the renal tissues. The study clearly shows that miR-I-LV significantly decreases the expression of miR-155 in kidneys compared to LPS. Administration of miR-I-LV remarkably reduced the pathological concerns of the kidneys with a marked decrease in inflammatory cell infiltration. Scrambled miR-155 did not have any effect on the expression of these markers; however miR-I-LV showed a remarkable ability to decrease the expression of TNF-α and IL-6 in kidney tissues indicating an ability to treat acute kidney infections. Overall, administration of miR-155 inhibitor effectively alleviated LPS-induced kidney injury by significantly suppressing TNF-α and IL-6 in kidney tissue and by remarkably increasing the expression of mRNA of SOCS1 and STAT1. The present results suggest that miR-155 inhibitor could be used in an effective targeting strategy for the treatment of acute kidney injury (AKI).
在本研究中，我们制备了负载miR - 155抑制剂的脂质体囊泡，用于有效治疗急性肾损伤。评估了脂质体miR - 155抑制剂在miR - 155表达、动物死亡率、TNF -α - IL6表达以及SOCS1 - STAT1表达方面的疗效。将miR - 155抑制剂负载到脂质体中赋予了其所需的胶体稳定性，并能有效递送至肾组织。该研究清楚地表明，与脂多糖（LPS）相比，miR - I - LV显著降低了肾脏中miR - 155的表达。给予miR - I - LV显著减轻了肾脏的病理问题，炎症细胞浸润明显减少。乱序miR - 155对这些标志物的表达没有任何影响；然而，miR - I - LV显示出显著降低肾组织中TNF -α和IL - 6表达的能力，表明其具有治疗急性肾脏感染的能力。总体而言，给予miR - 155抑制剂通过显著抑制肾组织中的TNF -α和IL - 6以及显著增加SOCS1和STAT1的mRNA表达，有效减轻了LPS诱导的肾损伤。目前的结果表明，miR - 155抑制剂可用于治疗急性肾损伤（AKI）的有效靶向策略。
