Ren Guojuan, Yu Liying, Zhu Baoya, Tang Mingyu, Chai Fang, Wang Chungang, Su Zhongmin
Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Colleges of Heilongjiang Province, College of Chemistry and Chemical Engineering, Harbin Normal University Harbin 150025 P. R. China
Faculty of Chemistry, Northeast Normal University Changchun 130024 P. R. China
RSC Adv. 2018 Apr 30;8(29):16095-16102. doi: 10.1039/c8ra01678j. eCollection 2018 Apr 27.
In this study, infrequent orange carbon nanodots (CNDs) were applied as a dual-readout probe for the effective colorimetric and fluorescent detection of 2,4,6-trinitrophenol (TNP). The orange fluorescence could be rapidly and selectively quenched by TNP, and the colorimetric response from the original pink color to blue could also be captured immediately by the naked eye. A limit of detection of 0.127 μM for TNP was estimated by the fluorescent method and 5 × 10 M by visualized detection. Interestingly, the fluorescence of the CNDs with TNP gradually transitioned from orange to green upon irradiation by a UV lamp, and the colorimetric response transitioned from pink to blue to colorless, which ensured effective multi-response detection of TNP. In addition, the CNDs exhibited bright fluorescence, excellent biocompatibility and low toxicity, making them high-quality fluorescent probes for cellular imaging.
在本研究中，稀有的橙色碳纳米点（CNDs）被用作双读出探针，用于对2,4,6-三硝基苯酚（TNP）进行有效的比色和荧光检测。橙色荧光可被TNP快速、选择性地猝灭，并且从原始粉红色到蓝色的比色响应也能立即被肉眼捕捉到。通过荧光法估计TNP的检测限为0.127 μM，通过可视化检测为5×10⁻⁶ M。有趣的是，在紫外灯照射下，含有TNP的CNDs的荧光从橙色逐渐转变为绿色，比色响应从粉红色转变为蓝色再变为无色，这确保了对TNP的有效多响应检测。此外，CNDs表现出明亮的荧光、优异的生物相容性和低毒性，使其成为用于细胞成像的高质量荧光探针。
