Li Juehong, Li Yamin, Peng Xiaochun, Li Bin, Qin Hui, Chen Yunsu
Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai China
RSC Adv. 2018 Jan 30;8(10):5151-5157. doi: 10.1039/c7ra12325f. eCollection 2018 Jan 29.
Metal wear particles play a major role in periprosthetic osteolysis and aseptic loosening in patients with total joint arthroplasty. The ability to induce osteolysis depends on the size, shape, dose, and type of the particles. However, much remains unknown regarding which type of metal particles are most reactive. We compared the inflammatory response and bone loss induced by two metal wear particles, cobalt-chromium-molybdenum (CoCrMo) and titanium (Ti), in a mouse calvaria model of osteolysis. We found that CoCrMo particles caused markedly greater bone resorption than Ti particles, according to three-dimensional images of the calvariae. CoCrMo particles activated more functional osteoclasts by significantly increasing the expression of the osteoclast-specific gene tartrate-specific acid phosphatase (Trap), calcitonin receptor (Ctr), and nuclear factor of activated T cells c1 (Nfatc1), and induced a greater increase in the ratio of receptor activator of nuclear factor kappa B ligand (RANKL)/osteoprotegerin (OPG) than Ti particles. CoCrMo particles also induced a stronger local inflammatory response, markedly increasing the expression and secretion of tumor necrosis factor-α and interleukin-1β compared with Ti particles. Therefore, CoCrMo particles induced a more severe inflammatory response and greater osteolysis than Ti particles .
金属磨损颗粒在全关节置换患者的假体周围骨溶解和无菌性松动中起主要作用。诱导骨溶解的能力取决于颗粒的大小、形状、剂量和类型。然而，关于哪种类型的金属颗粒反应性最强，仍有许多未知之处。我们在小鼠颅骨骨溶解模型中比较了两种金属磨损颗粒，钴铬钼（CoCrMo）和钛（Ti），所诱导的炎症反应和骨质流失。根据颅骨的三维图像，我们发现CoCrMo颗粒比Ti颗粒引起的骨吸收明显更多。CoCrMo颗粒通过显著增加破骨细胞特异性基因酒石酸特异性酸性磷酸酶（Trap）、降钙素受体（Ctr）和活化T细胞核因子c1（Nfatc1）的表达，激活了更多的功能性破骨细胞，并且比Ti颗粒诱导核因子κB受体活化因子配体（RANKL）/骨保护素（OPG）的比值有更大的增加。CoCrMo颗粒还诱导了更强的局部炎症反应，与Ti颗粒相比，显著增加了肿瘤坏死因子-α和白细胞介素-1β的表达和分泌。因此，CoCrMo颗粒比Ti颗粒诱导了更严重的炎症反应和更大程度的骨溶解。
