Biancone L, Wise L S, Das K M
Department of Medicine, University of Medicine of New Jersey-Robert Wood Johnson Medical School, New Brunswick 08903.
Gut. 1991 May;32(5):504-8. doi: 10.1136/gut.32.5.504.
In patients with ulcerative colitis a colon tissue bound IgG and serum antibodies against an Mr 40,000 colonic protein(s) has been identified. Using an anti-Mr 40,000 protein monoclonal antibody, 7E12H12, by an immunocytochemical method, the protein was localised in human tissue exclusively to colonic epithelial cells. In this study the presence of the Mr 40,000 protein was assessed in experimental animals by the direct and inhibition enzyme linked immunosorbent assays (ELISAs) using the anti-Mr 40,000 protein monoclonal antibody, 7E12H12 (IgM isotype). In addition, a total of 129 specimens including colon, small intestine, gall bladder, biliary tract, and kidney from nine strains of rats and mice, and from human tissue were studied by the immunocytochemical method using 7E12H12. All colon specimens from both humans and animals reacted with 7E12H12 in the immunocytochemical and ELISA assays. None of the non-colonic organs reacted with 7E12H12. While in human colon 7E12H12 recognised the absorptive epithelial cells, in all the animals it recognised mainly the colonic goblet cells. Extracts of animal colon but not of small intestine inhibited the binding of 7E12H12 to the human colon extract. This study shows the presence of an organ specific Mr 40,000 colonic epithelial protein(s) in humans and experimental animals. A differing cellular localisation of the Mr 40,000 protein(s) in human v animal tissue was also shown. Further characterisation of the Mr 40,000 protein(s) may provide important clues regarding the autoimmune mechanisms in ulcerative colitis.
在溃疡性结肠炎患者中，已鉴定出结肠组织中结合的IgG以及针对分子量为40,000的结肠蛋白的血清抗体。使用抗分子量为40,000蛋白的单克隆抗体7E12H12，通过免疫细胞化学方法，该蛋白在人体组织中仅定位于结肠上皮细胞。在本研究中，使用抗分子量为40,000蛋白的单克隆抗体7E12H12（IgM同种型），通过直接和抑制酶联免疫吸附测定（ELISA）评估实验动物中分子量为40,000蛋白的存在。此外，使用7E12H12通过免疫细胞化学方法研究了来自9种大鼠和小鼠品系以及人体组织的总共129个标本，包括结肠、小肠、胆囊、胆道和肾脏。来自人和动物的所有结肠标本在免疫细胞化学和ELISA测定中均与7E12H12反应。非结肠器官均未与7E12H12反应。在人类结肠中，7E12H12识别吸收性上皮细胞，而在所有动物中，它主要识别结肠杯状细胞。动物结肠提取物而非小肠提取物抑制7E12H12与人结肠提取物的结合。本研究表明在人和实验动物中存在一种器官特异性的分子量为40,000的结肠上皮蛋白。还显示了分子量为40,000蛋白在人类与动物组织中的细胞定位差异。对分子量为40,000蛋白的进一步表征可能为溃疡性结肠炎的自身免疫机制提供重要线索。
