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一种常见的正向反式作用因子与小鼠H-2和β2-微球蛋白基因启动子中的增强子序列结合。

A common positive trans-acting factor binds to enhancer sequences in the promoters of mouse H-2 and beta 2-microglobulin genes.

作者信息

Israël A, Kimura A, Kieran M, Yano O, Kanellopoulos J, Le Bail O, Kourilsky P

出版信息

Proc Natl Acad Sci U S A. 1987 May;84(9):2653-7. doi: 10.1073/pnas.84.9.2653.

DOI:10.1073/pnas.84.9.2653
PMID:3554244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC304716/
Abstract

Using gel retardation and in vitro "footprinting", we have analyzed the interactions between nuclear proteins derived from various mouse cells and the enhancer and interferon response sequences of the H-2Kb gene. We have found that a protein factor binds a site in the enhancer sequence that partially overlaps the interferon response sequence. This factor also binds to a similar sequence lying in the opposite orientation in the promoter of the mouse beta 2-microglobulin gene, suggesting a common regulatory mechanism. Transfection competition experiments indicate that this factor acts as a positive element in the expression of H-2 and beta 2-microglobulin genes.

摘要

利用凝胶阻滞和体外“足迹”技术，我们分析了源自各种小鼠细胞的核蛋白与H-2Kb基因的增强子及干扰素反应序列之间的相互作用。我们发现一种蛋白因子结合在增强子序列中的一个位点上，该位点与干扰素反应序列部分重叠。这种因子还与小鼠β2-微球蛋白基因启动子中呈相反方向的类似序列结合，提示存在一种共同的调控机制。转染竞争实验表明，这种因子在H-2和β2-微球蛋白基因的表达中起正性元件的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/dd160a933100/pnas00274-0100-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/535a3008c42f/pnas00274-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/9ed8da1b02fd/pnas00274-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/d0308447de5b/pnas00274-0100-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/2b63d37c582d/pnas00274-0100-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/dd160a933100/pnas00274-0100-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/535a3008c42f/pnas00274-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/9ed8da1b02fd/pnas00274-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/d0308447de5b/pnas00274-0100-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/2b63d37c582d/pnas00274-0100-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a326/304716/dd160a933100/pnas00274-0100-d.jpg

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A common positive trans-acting factor binds to enhancer sequences in the promoters of mouse H-2 and beta 2-microglobulin genes.一种常见的正向反式作用因子与小鼠H-2和β2-微球蛋白基因启动子中的增强子序列结合。
Proc Natl Acad Sci U S A. 1987 May;84(9):2653-7. doi: 10.1073/pnas.84.9.2653.
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本文引用的文献

1
The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.人类Daudi细胞中的β2-微球蛋白信使核糖核酸有一个突变的起始密码子，但仍可被干扰素诱导。
EMBO J. 1983;2(2):239-43. doi: 10.1002/j.1460-2075.1983.tb01412.x.
2
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
3
Control of HLA-A,B,C synthesis and expression in interferon-treated cells.
在I类主要组织相容性复合体基因启动子中鉴定出一个对逆转录病毒序列激活有反应的顺式作用元件。
J Virol. 1997 Feb;71(2):965-70. doi: 10.1128/JVI.71.2.965-970.1997.
4
Identification of a novel NF-kappaB p50-related protein in B lymphocytes.B淋巴细胞中一种新型NF-κB p50相关蛋白的鉴定。
Mol Cell Biol. 1996 Dec;16(12):7089-97. doi: 10.1128/MCB.16.12.7089.
5
The bcl-3 proto-oncogene encodes a nuclear I kappa B-like molecule that preferentially interacts with NF-kappa B p50 and p52 in a phosphorylation-dependent manner.bcl-3原癌基因编码一种核内类IκB分子，该分子以磷酸化依赖的方式优先与NF-κB p50和p52相互作用。
Mol Cell Biol. 1993 Jun;13(6):3557-66. doi: 10.1128/mcb.13.6.3557-3566.1993.
6
KBF1 (p50 NF-kappa B homodimer) acts as a repressor of H-2Kb gene expression in metastatic tumor cells.KBF1（p50核因子κB同型二聚体）在转移性肿瘤细胞中作为H-2Kb基因表达的抑制因子发挥作用。
J Exp Med. 1993 Jun 1;177(6):1651-62. doi: 10.1084/jem.177.6.1651.
7
The inducible transcription factor NF-kappa B: structure-function relationship of its protein subunits.诱导型转录因子核因子-κB：其蛋白质亚基的结构-功能关系
Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):297-308. doi: 10.1042/bj2900297.
8
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Mol Cell Biol. 1993 Mar;13(3):1315-22. doi: 10.1128/mcb.13.3.1315-1322.1993.
9
N-myc suppresses major histocompatibility complex class I gene expression through down-regulation of the p50 subunit of NF-kappa B.
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干扰素处理细胞中HLA - A、B、C合成与表达的调控
EMBO J. 1982;1(3):345-9. doi: 10.1002/j.1460-2075.1982.tb01172.x.
4
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Cell. 1983 Aug;34(1):189-96. doi: 10.1016/0092-8674(83)90149-6.
5
Preferential effect of gamma interferon on the synthesis of HLA antigens and their mRNAs in human cells.γ干扰素对人细胞中HLA抗原及其mRNA合成的优先作用。
Nature. 1982 Oct 28;299(5886):833-6. doi: 10.1038/299833a0.
6
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7
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8
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Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
9
Protein-DNA recognition.蛋白质-脱氧核糖核酸识别
Annu Rev Biochem. 1984;53:293-321. doi: 10.1146/annurev.bi.53.070184.001453.
10
Methidiumpropyl-EDTA.Fe(II) and DNase I footprinting report different small molecule binding site sizes on DNA.甲基丙基-乙二胺四乙酸铁（II）和脱氧核糖核酸酶I足迹法揭示了DNA上不同的小分子结合位点大小。
Nucleic Acids Res. 1983 Aug 25;11(16):5555-67. doi: 10.1093/nar/11.16.5555.