Rosa F, Berissi H, Weissenbach J, Maroteaux L, Fellous M, Revel M
Institut Pasteur, Université Paris VII, France.
EMBO J. 1983;2(2):239-43. doi: 10.1002/j.1460-2075.1983.tb01412.x.
The human Burkitt lymphoma cell line Daudi does not synthesize beta2-microglobulin (beta2m) and lacks the cell surface histocompatibility antigens. The cells, however, contain RNA hybridizing to a cloned human beta2m cDNA probe. cDNA from this Daudi beta2m RNA, was cloned and sequenced. By comparison with cDNA prepared from Ramos cells, which synthesized microglobulin, we determined the sequence of the 20 amino acid long leader peptide of pre-beta2m and show that in Daudi cells the initiator ATG has been mutated to ATC. Although Daudi beta2m RNA cannot be translated, interferon induces the beta2m RNA in Daudi cells as well as in normal human cells.
人类伯基特淋巴瘤细胞系Daudi不合成β2-微球蛋白（β2m），且缺乏细胞表面组织相容性抗原。然而，这些细胞含有与克隆的人类β2m cDNA探针杂交的RNA。从该Daudiβ2m RNA制备的cDNA被克隆并测序。通过与合成微球蛋白的Ramos细胞制备的cDNA进行比较，我们确定了前β2m的20个氨基酸长前导肽的序列，并表明在Daudi细胞中起始密码子ATG已突变为ATC。尽管Daudiβ2m RNA不能被翻译，但干扰素可诱导Daudi细胞以及正常人类细胞中的β2m RNA。
