Xu Qingsong, Qu Chen, Wan Jin, Cheng Gong, Yang Wen, Gong Changhao, He Jun, Du Yuguang
Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University 52. Heishijiao Street, Shahekou District Dalian 116023 China
Institute of Animal Nutrition, Sichuan Agricultural University Chengdu 611130 China
RSC Adv. 2018 Apr 10;8(24):13266-13273. doi: 10.1039/c7ra10172d. eCollection 2018 Apr 9.
Fecundity improvement is one of the most important economic traits for the swine industry as it significantly increases production efficiency. Intriguingly, chitosan oligosaccharide (COS), a biomaterial with an active amino group, could promote sow reproductive performance. Therefore, we investigated the effects of dietary COS supplementation on the gene expression differences in the ovaries of sows using the RNA-Seq method. This analysis obtained 13 960 051 and 14 564 863 clean reads in control ovary and COS ovary libraries, respectively. A total of 486 differentially expressed genes (DEGs) were thereby identified (FDR ≤ 0.001, |log ratio| ≥ 1). There were 234 up-regulated and 252 down-regulated genes in the COS ovary samples compared with the control ovary samples. A large number of these DEGs were involved in the terms cellular process, cell & cell part and binding. Furthermore, pathway analysis indicated that these DEGs were significantly enriched in 34 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including cell cycle, progesterone-mediated oocyte maturation, metabolic pathways, oocyte meiosis, and hematopoietic cell lineage among others. These results provided the molecular mechanisms of using COS feed additive for improving sow litter size and prolificacy.
繁殖力提高是养猪业最重要的经济性状之一，因为它能显著提高生产效率。有趣的是，壳寡糖（COS）作为一种具有活性氨基的生物材料，可促进母猪的繁殖性能。因此，我们使用RNA测序方法研究了日粮添加COS对母猪卵巢基因表达差异的影响。该分析在对照卵巢文库和COS卵巢文库中分别获得了13960051和14564863条clean reads。由此共鉴定出486个差异表达基因（FDR≤0.001，|log比值|≥1）。与对照卵巢样本相比，COS卵巢样本中有234个上调基因和252个下调基因。这些差异表达基因大量涉及细胞过程、细胞和细胞部分以及结合等术语。此外，通路分析表明，这些差异表达基因在34条京都基因与基因组百科全书（KEGG）通路中显著富集，包括细胞周期、孕酮介导的卵母细胞成熟、代谢通路、卵母细胞减数分裂和造血细胞谱系等。这些结果为使用COS饲料添加剂提高母猪产仔数和繁殖力提供了分子机制。
