Du Zhe, Wang Shujun, Wang You
Department of Bone and Joint Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University 145 Middle Shandong Road Shanghai China 200001
Department of Immunology, Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine Shanghai China.
RSC Adv. 2018 Jan 9;8(4):1866-1874. doi: 10.1039/c7ra10589d. eCollection 2018 Jan 5.
The efficacy of polyether-ether-ketone (PEEK) as a bearing material in knee components, a potential alternative to the currently used highly cross-linked polyethylene (HXLPE), has attracted a lot of attention recently. This study aimed to systematically assess the effect of particulate wear debris on CD4 and CD8 T-cell responses. HXLPE and PEEK particles (96% less than 5 μm) were generated by custom cryo-milling and pulverization in liquid nitrogen, and then incubated with blood collected from 25 donors. The phenotypes of the T-cells were systematically analyzed by immunostaining and flow cytometry. For the study, 0.1 mL of each particle suspension (about 1.0 × 10 wear particles) was injected into murine knee joints; the synovium and spleen were collected one week later for histological examination and immunofluorescence staining. PEEK and HXLPE particles did not induce CD4+ T-cell responses; however, CD8+ T-cells might be involved in mediating particle-induced reactions. The T-cell and inflammatory responses induced by PEEK and HXLPE particles were comparable. Further investigations into the frictional properties of materials should be performed to expand on our results.
聚醚醚酮（PEEK）作为膝关节部件的一种轴承材料，是目前使用的高度交联聚乙烯（HXLPE）的一种潜在替代品，其功效最近引起了广泛关注。本研究旨在系统评估颗粒磨损碎片对CD4和CD8 T细胞反应的影响。通过定制的低温研磨和在液氮中粉碎产生HXLPE和PEEK颗粒（96%小于5μm），然后与从25名供体采集的血液一起孵育。通过免疫染色和流式细胞术系统分析T细胞的表型。在该研究中，将0.1 mL每种颗粒悬浮液（约1.0×10磨损颗粒）注射到小鼠膝关节中；一周后收集滑膜和脾脏进行组织学检查和免疫荧光染色。PEEK和HXLPE颗粒未诱导CD4 + T细胞反应；然而，CD8 + T细胞可能参与介导颗粒诱导的反应。PEEK和HXLPE颗粒诱导的T细胞和炎症反应相当。应进一步研究材料的摩擦性能以扩展我们的研究结果。
