Li Mengyao, Zhai Chen, Wang Shuya, Huang Weixia, Liu Yunguo, Li Zhao
Nutrition & Health Research Institute, COFCO Corporation, Beijing Key Laboratory of Nutrition & Health and Food Safety Beijing 102209 China
College of Life Science and Technology, Xinjiang University Urumqi 830002 China.
RSC Adv. 2019 Dec 9;9(69):40689-40693. doi: 10.1039/c9ra08150j. eCollection 2019 Dec 3.
A novel hydrosoluble near-infrared fluorescence off-on probe has been developed for detecting carboxylesterase activity. The probe was designed by introducing (4-acetoxybenzyl)oxy as a quenching and recognizing moiety to the decomposed product of IR-783, which exhibits excellent near-infrared fluorescence features and good water solubility. The responding mechanism of novel probe 1 to carboxylesterase was investigated. It would lead to the cleavage of the carboxylic ester bond by carboxylesterase catalyze the spontaneous hydrolysis of the probe, resulting in the release of a near-infrared fluorophore. This behaviour leads to the development of a simple and sensitive fluorescent method for assaying carboxylesterase activity, with a detection limit of 3.4 × 10 U mL. Moreover, the probe displays excellent selectivity toward carboxylesterase over other substances. Notably, the imaging experimental results showed that the probe 1 is cell membrane permeable, and its applicability has been demonstrated for monitoring carboxylesterase activity in HeLa cells.
一种新型水溶性近红外荧光开-关型探针已被开发用于检测羧酸酯酶活性。该探针是通过将(4-乙酰氧基苄基)氧基作为猝灭和识别部分引入到IR-783的分解产物中而设计的，IR-783具有优异的近红外荧光特性和良好的水溶性。研究了新型探针1对羧酸酯酶的响应机制。羧酸酯酶会催化探针的自发水解，导致羧酸酯键断裂，从而释放出近红外荧光团。这种行为促成了一种用于测定羧酸酯酶活性的简单灵敏的荧光方法的开发，检测限为3.4×10 U/mL。此外，该探针对羧酸酯酶显示出优于其他物质的优异选择性。值得注意的是，成像实验结果表明探针1具有细胞膜通透性，并且已证明其在监测HeLa细胞中羧酸酯酶活性方面的适用性。
