Wang Miao, Li Minmin, Lu Jia, Fan Bei, He Yan, Huang Yatao, Wang Fengzhong
Key Laboratory of Quality & Safety Risk Assessment on Agro-Products Processing (Beijing), Ministry of Agriculture Beijing 100193 China
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences Beijing 100193 China.
RSC Adv. 2018 Mar 23;8(21):11551-11556. doi: 10.1039/c7ra13404e. eCollection 2018 Mar 21.
Herein, we report novel "off-on" fluorescent sensing of organophosphate pesticides using a carbon dot (CD)-Au(iii) complex/acetylcholinesterase (AChE) system. The above sensor utilizes the quenching of CD fluorescence by Au(iii) and its subsequent recovery by thiocholine, which is generated by AChE-catalyzed hydrolysis of acetylthiocholine (ATCh) and effectively scavenges Au(iii). In the presence of organophosphates, the catalytic activity of AChE is inhibited, allowing these species to be quantified based on the decreased recovery of CD fluorescence intensity. The developed sensor was used to analyze a real pesticide-spiked sample (4.48 μM), achieving a recovery of 99.85% and exhibiting a linear response range of 0.45-44.80 μM.
在此，我们报道了一种利用碳点（CD）-金（III）配合物/乙酰胆碱酯酶（AChE）系统对有机磷酸酯农药进行新型“开-关”荧光传感的方法。上述传感器利用金（III）对CD荧光的猝灭以及随后硫代胆碱使其恢复荧光，硫代胆碱是由AChE催化乙酰硫代胆碱（ATCh）水解产生的，能有效清除金（III）。在有机磷酸酯存在的情况下，AChE的催化活性受到抑制，从而可根据CD荧光强度恢复的降低对这些物质进行定量分析。所开发的传感器用于分析实际添加农药的样品（4.48 μM），回收率达到99.85%，线性响应范围为0.45 - 44.80 μM。
