College of Chemistry and Materials Science, Anhui Normal University , Wuhu, Anhui 241000, China.
Anal Chem. 2014 Jun 3;86(11):5323-9. doi: 10.1021/ac5001338. Epub 2014 May 22.
In this paper, we have presented a novel strategy to fabricate fluorescent boronic acid modified carbon dots (C-dots) for nonenzymatic blood glucose sensing applications. The functionalized C-dots are obtained by one-step hydrothermal carbonization, using phenylboronic acid as the sole precursor. Compared with conventional two-step fabrication of nanoparticle-based sensors, the present "synthesis-modification integration" strategy is simpler and more efficient. The added glucose selectively leads to the assembly and fluorescence quenching of the C-dots. Such fluorescence responses can be used for well quantifying glucose in the range of 9-900 μM, which is 10-250 times more sensitive than that of previous boronic acid based fluorescent nanosensing systems. Due to "inert" surface, the C-dots can well resist the interferences from various biomolecules and exhibit excellent selectivity. The proposed sensing system has been successfully used for the assay of glucose in human serum. Due to simplicity and effectivity, it exhibits great promise as a practical platform for blood glucose sensing.
在本文中，我们提出了一种新的策略，用于制造荧光硼酸修饰的碳点（C-dots），用于非酶血糖传感应用。功能化的 C-dots 通过一步水热碳化获得，使用苯硼酸作为唯一的前体。与基于纳米粒子的传感器的传统两步制造相比，本“合成-修饰集成”策略更简单、更高效。添加的葡萄糖选择性地导致 C-dots 的组装和荧光猝灭。这种荧光响应可用于在 9-900 μM 的范围内很好地定量葡萄糖，比以前基于硼酸的荧光纳米传感系统灵敏 10-250 倍。由于“惰性”表面，C-dots 可以很好地抵抗各种生物分子的干扰，并表现出优异的选择性。所提出的传感系统已成功用于人血清中葡萄糖的测定。由于其简单性和有效性，它有望成为一种实用的血糖传感平台。
