Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
RECETOX, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Chem Commun (Camb). 2022 May 30;58(44):6389-6392. doi: 10.1039/d2cc02016e.
Many serious diseases are associated with degenerative changes caused by oxidative stress triggered by elevated concentrations of reactive oxygen species (ROS) in cells. Therefore, the development of suitable probes for monitoring such processes is of great importance. Here, we introduce a series of sulfur- and selenium-substituted BODIPY derivatives as reversible redox sensors for ROS and enzymatic redox processes. Significant differences in emission maxima and fluorescence quantum yields between the reduced and oxidized forms make them excellent ratiometric turn-on/off probes. Installation of polar sulfonate groups improved their aqueous solubility while retaining their sensing properties, which allowed the probes to monitor the enzymatic activity of enantioselective methionine sulfoxide reductase.
许多严重的疾病都与氧化应激引起的退行性变化有关，而氧化应激是由细胞内活性氧（ROS）浓度升高引起的。因此，开发合适的探针来监测这些过程非常重要。在这里，我们介绍了一系列硫代和硒代 BODIPY 衍生物，它们可用作 ROS 和酶促氧化还原过程的可逆氧化还原传感器。还原和氧化形式之间的发射最大值和荧光量子产率的显著差异使它们成为出色的比率型开/关探针。极性磺酸盐基团的引入提高了它们的水溶性，同时保留了它们的传感性能，这使得这些探针能够监测手性甲硫氨酸亚砜还原酶的酶活性。
