National Centre for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
Al-Azhar University, Cairo, Egypt.
Integr Cancer Ther. 2022 Jan-Dec;21:15347354221096668. doi: 10.1177/15347354221096668.
The rising interest in innovative methods of cancer immunotherapy has prompted research into the immunomodulatory mechanisms of natural and synthetic substances. The goal of this study was to assess chrysin immune-stimulating and pro-apoptotic effects on tumor growth and cell susceptibility to ionizing radiation in order to improve cancer therapy. Chrysin (20 mg/kg/day) was intraperitoneally injected to mice bearing 1 cm solid tumor of Ehrlich ascites carcinoma (EAC) for 21 consecutive days. Mice were whole body exposed to 1 Gy of gamma radiation (2 fractionated dose 0.5 Gy each). Treatment with chrysin dramatically reduces tumor proliferation in EAC mice; furthermore, IFN-γ activity is significantly reduced when compared to EAC mice. When compared to EAC mice, the expression of TNF-α, free radicals, and nitric oxide (NO) levels were considerably reduced, along with improvements in apoptotic regulators (caspase-3 activity). Moreover, the histopathological investigation confirms the improvement exerted by chrysin even in the EAC mice group or the EAC + R group. What is more, exposure to gamma radiation sustained the modulatory effect of chrysin on tumor when compared with EAC + Ch mice. Hence, chrysin might represent a potential therapeutic strategy for increasing the radiation response of solid tumor.
人们对癌症免疫疗法的创新方法越来越感兴趣，这促使人们研究天然和合成物质的免疫调节机制。本研究的目的是评估白杨素对肿瘤生长和细胞对电离辐射敏感性的免疫刺激和促凋亡作用，以改善癌症治疗。用 20mg/kg/天的白杨素腹腔注射接种 1cm 固体艾氏腹水癌（EAC）的小鼠，连续 21 天。用 1Gyγ射线对小鼠进行全身照射（2 次分割剂量为 0.5Gy）。白杨素治疗可显著降低 EAC 小鼠的肿瘤增殖；与 EAC 小鼠相比，IFN-γ 活性显著降低。与 EAC 小鼠相比，TNF-α、自由基和一氧化氮（NO）水平的表达明显降低，同时凋亡调节剂（caspase-3 活性）也得到改善。此外，组织病理学研究证实，即使在 EAC 小鼠组或 EAC+R 组中，白杨素也能发挥改善作用。更重要的是，与 EAC+Ch 小鼠相比，γ 射线照射维持了白杨素对肿瘤的调节作用。因此，白杨素可能代表一种增加实体瘤对辐射反应的潜在治疗策略。
