Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Jiangsu Provincial Key Laboratory of Critical Care Medicine, Key Laboratory of Environmental Medicine Engineering, Ministry of Education, State Key Laboratory of Bioelectronics, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Anal Chem. 2022 May 24;94(20):7350-7357. doi: 10.1021/acs.analchem.2c00785. Epub 2022 May 11.
Inspired by the promising applications of a closed bipolar electrodes (-BPEs) system in electrochemiluminescence (ECL) detection of cell adhesion and disease-related biomarkers, here, a gold nanowires array-based -BPEs system was constructed for cell surface protein detection. Regular and uniform gold nanowires array were prepared by intermittent potentiostatic deposition. Then, two poly(dimethylsiloxane) (PDMS) chips with a hole diameter of 2 mm as a reservoir were placed at both sides of Au nanowires array to construct -BPEs system. Thionine-functionalized silicon dioxide nanoparticles conjugated to antibody (Ab2-Th@SiO) were used as the electrochemical probe, while [Ru(bpy)]-wrapped SiO nanoparticles (Ru(II)@SiO) were employed as the ECL signal readout. Taking α-fetoprotein (AFP) as model, the gold nanowires array-based -BPEs system allowed sensitive detection of AFP at a linear range from 0.002 to 50.0 ng/mL and at least 6 living cells ascribing to the synergetic amplification effect at both sensing and reporting chambers. Besides, the amount of AFP expressed by HepG2 cells was calculated to be 6.71 pg/cell. The presented strategy with high sensitivity provided a promising and universal platform for the detection of other cancer cells and disease-related biomarkers (such as proteins, glycan, miRNA).
受闭环比双极电极 (-BPEs) 系统在细胞黏附及疾病相关生物标志物电化学发光 (ECL) 检测中应用的启发，本研究构建了一种基于金纳米线阵列的 -BPEs 系统用于细胞表面蛋白检测。通过间歇恒电位沉积制备了规则且均匀的金纳米线阵列。然后，将两个具有 2mm 孔径的聚二甲基硅氧烷 (PDMS) 芯片置于 Au 纳米线阵列的两侧，以构建 -BPEs 系统。将与抗体（Ab2-Th@SiO）偶联的噻吨基功能化二氧化硅纳米粒子用作电化学探针，同时将[Ru(bpy)]包裹的 SiO 纳米粒子（Ru(II)@SiO）用作 ECL 信号读出。以甲胎蛋白（AFP）为模型，基于金纳米线阵列的 -BPEs 系统在 0.002 至 50.0ng/mL 的线性范围内对 AFP 进行了灵敏检测，并且至少可以检测到 6 个活细胞，这归因于在传感和报告腔室中的协同放大效应。此外，计算得出 HepG2 细胞表达的 AFP 量为 6.71pg/细胞。该高灵敏度的方法为检测其他癌细胞和疾病相关生物标志物（如蛋白质、聚糖、miRNA）提供了一个有前途且通用的平台。
