Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Jiangsu Provincial Key Laboratory of Critical Care Medicine, Key Laboratory of Environmental Medicine Engineering, Ministry of Education, State Key Laboratory of Bioelectronics, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
ACS Sens. 2022 Aug 26;7(8):2446-2453. doi: 10.1021/acssensors.2c01195. Epub 2022 Jul 25.
The integration of a closed bipolar electrode (-BPE) array and electrochemiluminescence (ECL) detection received a boost in applications in the detection of cell adhesion and disease-related biomarkers. This work proposed a gold nanorod array based -BPE-ECL system to realize an image of endogenous hydrogen peroxide (HO) efflux from living cells and parallel analysis of endogenous HO released from multiple cells by converting electrochemical signals into optical signals. The gold nanorod array with high density was prepared by a repeating chronopotentiometry procedure with anodic aluminum oxide (AAO) membrane as a template. The -BPE array was fabricated by assembling poly(dimethylsiloxane) (PDMS) chips on both sides of the gold nanorod array. When an appropriate driving potential is applied, HO generated from living cells at the sensing pole was reduced on the gold nanorod, triggering the oxidation of the ECL reagent at the reporting pole, which allowed the detection of HO released from living cells. Under phorbol myristate acetate (PMA) stimulation, HO released from living HeLa, HepG2, MCF-7, and LO2 cells was determined to be 47, 32.4, 25.7, and 6.3 μM, respectively. This indicated that the amount of HO released from PMA-stimulated cancer cells was significantly higher than that from the stimulated normal cells. This work presented a new approach for imaging of HO released from living cells and could also be used to detect other electrochemically active or non-electrochemically active molecules through simple cell surface modification, which may have potential applications in cell apoptosis study and disease diagnosis.
基于金纳米棒阵列的闭体型双极电极-电化学发光（BPE-ECL）系统用于实现活细胞内源性过氧化氢（HO）溢出的图像，并通过将电化学信号转化为光学信号来平行分析来自多个细胞的内源性 HO 的释放。使用具有模板的阳极氧化铝（AAO）膜通过重复恒电流程序制备具有高密度的金纳米棒阵列。通过将聚二甲基硅氧烷（PDMS）芯片组装在金纳米棒阵列的两侧来制造 BPE 阵列。当施加适当的驱动电势时，在传感极处来自活细胞的 HO 在金纳米棒上被还原，触发在报告极处的 ECL 试剂的氧化，从而允许检测来自活细胞的 HO 的释放。在佛波醇肉豆蔻酸酯（PMA）刺激下，来自活的 HeLa、HepG2、MCF-7 和 LO2 细胞的 HO 释放量分别为 47、32.4、25.7 和 6.3 μM。这表明 PMA 刺激的癌细胞释放的 HO 量明显高于受刺激的正常细胞。本工作提出了一种用于活细胞内源性 HO 释放成像的新方法，并且还可以通过简单的细胞表面修饰来检测其他电化学活性或非电化学活性分子，这可能在细胞凋亡研究和疾病诊断中有潜在应用。
