Department of Radiology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
The Affiliated Hospital of Southwest Medical University, Luzhou, China.
Photodiagnosis Photodyn Ther. 2022 Sep;39:102881. doi: 10.1016/j.pdpdt.2022.102881. Epub 2022 May 8.
Background Ultrasound, computed tomographic peritoneography, methylene blue, and peritoneal scintigraphy are commonly used to identify peritoneal dialysis-related complications in clinical settings. This study aimed to investigate the diagnostic value of indocyanine green in peritoneal dialysis-related complications and to study the effect of indocyanine green on residual renal function and peritoneal function. Methods Twenty male Sprague-Dawley rats were used to establish models, including a pleural effusion model (A, n = 4), abdominal hernia model (B, n = 4), subcutaneous leakage model (C, n = 4), and control (D, n = 8). They were injected with a 20 mL mixture of peritoneal dialysate and indocyanine green at varying concentrations prepared for near-infrared fluorescence imaging. We compared the results of near-infrared-I and near-infrared-II imaging. Radiologists evaluated the image quality, morphology, and thickness of the peritoneum, and the residual renal function was assessed using haematoxylin and eosin staining. Results Lesions in each rat model group were observed by changing the body position and imaging parameters. Pathological kidney and peritoneal sections showed no changes. Meanwhile, near-infrared-I fluorescence imaging of ICG has a better signal-to-background ratio than near-infrared-II. Conclusion Near-infrared-I fluorescence imaging of ICG has a better SBR than near-infrared-II and it is sufficient for for diagnosing peritoneal dialysis-related complications and ICG has no impact on residual renal function and peritoneal function. This method has clinical application potential in promptly diagnosing peritoneal dialysis-related complications.
背景 超声、计算机断层扫描腹膜造影、亚甲蓝和腹膜闪烁扫描常用于临床识别腹膜透析相关并发症。本研究旨在探讨吲哚菁绿在腹膜透析相关并发症中的诊断价值，并研究吲哚菁绿对残余肾功能和腹膜功能的影响。方法 20 只雄性 Sprague-Dawley 大鼠用于建立模型，包括胸腔积液模型（A，n=4）、腹疝模型（B，n=4）、皮下渗漏模型（C，n=4）和对照组（D，n=8）。它们被注射不同浓度的腹膜透析液和吲哚菁绿混合物进行近红外荧光成像。我们比较了近红外 I 和近红外 II 成像的结果。放射科医生评估图像质量、形态和腹膜厚度，并用苏木精和伊红染色评估残余肾功能。结果 改变体位和成像参数后，观察到每个大鼠模型组的病变。病理肾和腹膜切片无变化。同时，ICG 的近红外 I 荧光成像具有比近红外 II 更好的信噪比。结论 ICG 的近红外 I 荧光成像具有比近红外 II 更好的 SBR，足以诊断腹膜透析相关并发症，且对残余肾功能和腹膜功能无影响。该方法在及时诊断腹膜透析相关并发症方面具有临床应用潜力。
