School of Environmental Engineering, Henan University of Technology, Zhengzhou 450001, China; School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, China.
School of Environmental Engineering, Henan University of Technology, Zhengzhou 450001, China.
Bioresour Technol. 2022 Jul;355:127288. doi: 10.1016/j.biortech.2022.127288. Epub 2022 May 12.
Immobilization technology with low maintenance is a promising alternative to enhance nitrate removal from water. In this study, washing rice drainage (RWD) was immobilized by poly(vinyl alcohol)/sodium alginate (PVA/SA) to obtain RWD-PVA/SA gel beads as inoculum for denitrification. When initial nitrate concentration was 50 mg N/L, nitrate was effectively removed at rates of 50-600 mg/(L∙d) using acetate as carbon source (C/N = 1.25). Arrhenius activation energy (Ea) of nitrate oxidoreductase was 28.64 kJ/mol for the RWD-PVA/SA gel beads. Temporal and spatial variation in microbial community structures were revealed along with RWD storage and in the reactors by Illumina high-throughput sequencing technology. RWD-PVA/SA gel beads has a simple (operational taxonomic units (OTUs) 〈100). Dechloromonas, Pseudomonas, Flavobacterium and Acidovorax were the most four dominant genera in the denitrification reactors inoculated with RWD-PVA/SA gel beads. This study provides an inoculum for denitrification with high nitrate removal performance and simple microbial community structures.
低维护固定化技术是增强水中硝酸盐去除的一种很有前途的替代方法。在本研究中，采用聚乙烯醇/海藻酸钠（PVA/SA）对洗米水（RWD）进行固定化，得到 RWD-PVA/SA 凝胶珠作为反硝化的接种物。当初始硝酸盐浓度为 50mgN/L 时，以乙酸盐作为碳源（C/N=1.25），硝酸盐的去除率在 50-600mg/（L·d）之间。RWD-PVA/SA 凝胶珠的硝酸盐氧化还原酶的阿伦尼乌斯活化能（Ea）为 28.64kJ/mol。通过 Illumina 高通量测序技术揭示了 RWD 储存过程中和反硝化反应器中微生物群落结构的时间和空间变化。RWD-PVA/SA 凝胶珠具有简单的（操作分类单元（OTUs）〈100）特征。反硝化反应器中接种 RWD-PVA/SA 凝胶珠时，最主要的四个属为 Dechloromonas、Pseudomonas、Flavobacterium 和 Acidovorax。本研究提供了一种具有高硝酸盐去除性能和简单微生物群落结构的反硝化接种物。
