一种对人移行细胞癌具有选择性的单克隆抗体的制备。

Production of a monoclonal antibody selective to human transitional cell carcinoma.

作者信息

Takahashi N, Koizumi Y, Kawaguchi T, Takahashi K, Suzuki T, Funyu T

出版信息

Tohoku J Exp Med. 1987 Feb;151(2):213-20. doi: 10.1620/tjem.151.213.

DOI:10.1620/tjem.151.213

Abstract

In order to detect the bladder tumor specific antigens, the monoclonal antibody (MoAb) No. 10 to human transitional cell carcinoma of the bladder (T.C.C.B.) was obtained. Hybridomas were prepared by cell fusion between the mouse myeloma cell line X 63 Ag 8.653 and the spleen cells of BALB/c mouse hyperimmune to the bladder cancer cells (grade 2) from a patient, and were cloned. Consequently 12 MoAb-producing clones were obtained for the panel screening by enzymeimmunoassay (EIA) and then 3 MoAbs (No. 10, 11 and 14) were selected for the testing of reactivity to bladder cancer cells from patients including normal epithelia. Finally No. 10 was selected as the most appropriate MoAb for this study and was determined IgM with kappa-light chains by EIA.

摘要

为了检测膀胱肿瘤特异性抗原，获得了针对人膀胱移行细胞癌（T.C.C.B.）的单克隆抗体（MoAb）No. 10。通过将小鼠骨髓瘤细胞系X 63 Ag 8.653与对一名患者的膀胱癌细胞（2级）高度免疫的BALB/c小鼠的脾细胞进行细胞融合制备杂交瘤，并进行克隆。结果通过酶免疫测定（EIA）获得了12个产生MoAb的克隆用于组筛选，然后选择3种MoAb（No. 10、11和14）检测对包括正常上皮在内的患者膀胱癌细胞的反应性。最后，No. 10被选为该研究最合适的MoAb，并通过EIA确定其为带有κ轻链的IgM。