Takahashi N, Takahashi S, Takahashi K, Suzuki T, Funyu T
J Urol. 1987 Jul;138(1):207-11. doi: 10.1016/s0022-5347(17)43046-1.
In order to detect bladder tumor specific antigens, monoclonal antibodies (MoAbs) to human transitional cell carcinoma of the bladder (TCCB) were obtained. Hybridomas were cloned after being prepared by cell fusion between mouse myeloma cell line X63Ag 8.653 and the spleen cells of BALB/c mice hyperimmune to the bladder cancer cells (grade 2) from a patient. Subsequently 12 MoAb-producing clones were obtained for the panel screening by enzyme immunoassay (EIA) and three MoAbs (no. 10, 11 and 14) were selected for reactivity to bladder cancer cells from patients, including normal epithelia. Finally No. 10 was selected as the most appropriate MoAb for this study and determined IgM with kappa-light chains by EIA. We accurately tested MoAb No. 10's reactivity with 71 malignant tumors by immunoperoxidase staining (IIP). No. 10 reacted to most TCCB cells (36/43), but did not react to any of the other tissues, including five normal epithelia, two brain tumors and three sarcomas. It was also shown by both IIP and immunofluorescent staining (IIF) that No. 10's reactivity was limited to the cancer cell membrane. These results suggest that the antigen recognized by MoAb No. 10 is one of the tumor-associated antigens and that some heterogeneity exists in its distribution.
为了检测膀胱肿瘤特异性抗原，获得了针对人膀胱移行细胞癌（TCCB）的单克隆抗体（MoAb）。通过小鼠骨髓瘤细胞系X63Ag 8.653与对一名患者的膀胱癌细胞（2级）超免疫的BALB/c小鼠的脾细胞进行细胞融合制备杂交瘤，之后进行克隆。随后通过酶免疫测定（EIA）获得12个产生MoAb的克隆用于组筛选，并选择了三种MoAb（第10、11和14号）用于检测对包括正常上皮在内的患者膀胱癌细胞的反应性。最后，第10号被选为该研究最合适的MoAb，并通过EIA确定其为带有κ轻链的IgM。我们通过免疫过氧化物酶染色（IIP）准确检测了第10号MoAb对71种恶性肿瘤的反应性。第10号对大多数TCCB细胞（36/43）有反应，但对任何其他组织均无反应，包括五种正常上皮、两种脑肿瘤和三种肉瘤。IIP和免疫荧光染色（IIF）均显示，第10号的反应性仅限于癌细胞膜。这些结果表明，第10号MoAb识别的抗原是肿瘤相关抗原之一，并且其分布存在一些异质性。
