Ernst R, Williams L, Dalbey M, Collins C, Pankey S
Ther Drug Monit. 1987;9(1):85-90. doi: 10.1097/00007691-198703000-00015.
EMIT tests are available for quantitative determination of the tricyclic antidepressants amitriptyline (AMI), nortriptyline (NORT), imipramine (IMI), and desipramine (DMI). An extraction step before analysis eliminates cross-reacting polar metabolites. Excellent correlation between EMIT and high-performance liquid chromatography (HPLC) has been previously established. Most published protocols for using EMIT reagents on COBAS-BIO centrifugal analyzers were designed for analytes present at 1-50 mg/L in serum. AMI, NORT, IMI, and DMI are usually present at far less than 1 mg/L. We describe a COBAS-BIO EMIT protocol for assaying these analytes. Patient sample correlation between COBAS-BIO EMIT and EMIT-AutoLab were greater than 0.99. Between-run precision for single-point determinations was comparable to SYVA AutoLab performance [less than or equal to 11% at 40 micrograms/L AMI, NORT, IMI (80 micrograms/L DMI), and less than or equal to 4% at 200 micrograms/L AMI, NORT, IMI (400 micrograms/L DMI)]. With stored-curve updating, working reagents were usable for at least 14 days (AMI) or 23 days (DMI).
放射免疫分析法（EMIT）可用于定量测定三环类抗抑郁药阿米替林（AMI）、去甲替林（NORT）、丙咪嗪（IMI）和地昔帕明（DMI）。分析前的萃取步骤可消除产生交叉反应的极性代谢物。此前已证实EMIT与高效液相色谱法（HPLC）之间具有良好的相关性。大多数已发表的在COBAS - BIO离心分析仪上使用EMIT试剂的方案是针对血清中浓度为1 - 50 mg/L的分析物设计的。AMI、NORT、IMI和DMI的浓度通常远低于1 mg/L。我们描述了一种用于检测这些分析物的COBAS - BIO EMIT方案。COBAS - BIO EMIT与EMIT - AutoLab之间患者样本的相关性大于0.99。单点测定的批间精密度与SYVA AutoLab的性能相当[在40微克/升的AMI、NORT、IMI（80微克/升的DMI）时小于或等于11%，在200微克/升的AMI、NORT、IMI（400微克/升的DMI）时小于或等于4%]。通过存储曲线更新，工作试剂至少可使用14天（AMI）或23天（DMI）。
