Pankey S, Collins C, Jaklitsch A, Izutsu A, Hu M, Pirio M, Singh P
Clin Chem. 1986 May;32(5):768-72.
We describe specific EMIT homogeneous enzyme immunoassays for amitriptyline, nortriptyline, imipramine, and desipramine in patients' serum samples. Before analysis, an easily performed extraction step involving the use of 500 microL of sample and a 1-mL disposable column eliminates cross-reacting polar metabolites. The range of the standard curve for the first three drugs is 25 to 250 micrograms/L, and for desipramine is 50 to 500 micrograms/L. Within-run and between-run CVs are less than 10% throughout the range of the assays. Results for patients' samples obtained by this method and by "high-performance" liquid chromatography compare well, showing a slope range of 0.94-1.04 and correlation coefficients ranging from 0.93 to 0.96, depending on the assay.
我们描述了针对患者血清样本中阿米替林、去甲替林、丙咪嗪和地昔帕明的特定EMIT均相酶免疫测定法。在分析之前，一个易于操作的提取步骤，即使用500微升样本和一个1毫升一次性柱，可消除交叉反应的极性代谢物。前三种药物的标准曲线范围为25至250微克/升，地昔帕明的标准曲线范围为50至500微克/升。在整个测定范围内，批内和批间变异系数均小于10%。通过该方法和“高效”液相色谱法获得的患者样本结果比较良好，根据测定方法不同，斜率范围为0.94 - 1.04，相关系数范围为0.93至0.96。
