Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
Department of Biomedical Technology, Federal University of Technology, Akure, Nigeria.
Biomarkers. 2022 Sep;27(6):587-598. doi: 10.1080/1354750X.2022.2077446. Epub 2022 May 24.
We investigated the effect of African eggplant (AE) ( L) and Black nightshade (BN) L) leaves; two tropical vegetables consumed by humans on behavioural, biochemical and histological indices in model of Alzheimer's disease (AD).
Transgenic flies expressing human Amyloid Precursor Protein (hAPP) and β-secretase (hBACE 1) were exposed to the pulverised leaf samples (0.1 and 1.0%) in their diets for fourteen days. Thereafter, the flies were assessed for their behavioural indices and routine histology of brain cells. Furthermore, fly head homogenates were assayed for β-amyloid level, activities of acetylcholinesterase (AChE) and β-secretase (BACE-1), as well as oxidative stress markers.
Result showed that the significantly lower ( < 0.05) behavioural parameters (survival, locomotor performance and memory index), higher AChE and BACE-1 activities, β-amyloid, ROS and lipid peroxidation levels, as well as reduced antioxidant indices observed in the AD flies, were significantly ameliorated ( < 0.05) in AD flies treated with the leaf samples.
This study has showed that leaves of AE and BN ameliorated behavioural and biochemical indices in AD flies via neural enzyme modulatory, and antioxidant mechanisms.
Hence, this study further justifies the neuroprotective properties of both AE and BN.
我们研究了非洲茄子（AE）和黑茄（BN）叶子对阿尔茨海默病（AD）模型中行为、生化和组织学指标的影响。这两种热带蔬菜都被人类食用。
表达人淀粉样前体蛋白（hAPP）和β-分泌酶（hBACE1）的转基因果蝇暴露于饮食中的粉碎叶样品（0.1%和 1.0%）中 14 天。然后，评估果蝇的行为指标和脑细胞常规组织学。此外，还测定了果蝇头部匀浆中的β-淀粉样蛋白水平、乙酰胆碱酯酶（AChE）和β-分泌酶（BACE-1）的活性以及氧化应激标志物。
结果表明，AD 果蝇的行为参数（存活率、运动表现和记忆指数）显著降低（<0.05），AChE 和 BACE-1 活性、β-淀粉样蛋白、ROS 和脂质过氧化水平升高，抗氧化指标降低，用叶片样品处理的 AD 果蝇得到显著改善（<0.05）。
本研究表明，AE 和 BN 的叶子通过神经酶调节和抗氧化机制改善了 AD 果蝇的行为和生化指标。
因此，本研究进一步证明了 AE 和 BN 的神经保护特性。
