Functional Foods, Nutraceuticals and Phytomedicine Unit, Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
Department of Biochemistry, University of Medical Sciences, Ondo, Nigeria.
J Diet Suppl. 2021;18(4):372-386. doi: 10.1080/19390211.2020.1772441. Epub 2020 Jun 4.
() and () have been used by the locals for the management of Alzheimer's disease (AD) but with scant scientific sources. The aim of this study is to assess the neuroprotective properties of and using transgenic flies (TDMF). The activity of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), as well as the antioxidant status of TDMF raised on a diet supplemented with and were determined. The flies were raised on a diet devoid of supplements or supplemented with or (1% and 5% inclusion) for 7 days. Afterward, AChE and BChE activities, as well as catalase and total thiol level, were determined. The reactive oxygen species (ROS) and malondialdehyde (MDA) levels were also determined in the flies raised on a diet devoid of supplement and on supplemented diets. Meanwhile, flies raised on and supplemented diets exhibited a significant reduction in the activity of AChE and BChE in comparison with the controls. Also, supplemented diets significantly ( < 0.05) enhance catalase activity and improve total thiol level, while ROS and MDA levels were observed to be reduced in all the flies raised on the supplemented diets in comparison with the controls. In summary, reduction in the activity of AChE and BChE, as well as improved antioxidant status in TDMF, could be some of the mechanisms through which and exhibit anti-AD properties. Nevertheless, exhibits a better neuroprotective effect than
（）和（）已被当地人用于治疗阿尔茨海默病（AD），但科学依据甚少。本研究旨在利用转基因 TDMF 评估（）和（）的神经保护特性。通过测定补充（）和（）饮食的 TDMF 的乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）的活性以及抗氧化状态来评估。将果蝇饲养在缺乏补充剂或补充 1%和 5%（）或（）的饮食中 7 天。之后，测定 AChE 和 BChE 活性以及过氧化氢酶和总巯基水平。还在缺乏补充剂和补充剂饮食中饲养的果蝇中测定活性氧（ROS）和丙二醛（MDA）水平。与对照组相比，在（）和补充饮食中饲养的果蝇的 AChE 和 BChE 活性显著降低。此外，补充饮食可显著（<0.05）提高过氧化氢酶活性和改善总巯基水平，而在补充饮食中饲养的所有果蝇的 ROS 和 MDA 水平均比对照组降低。总之，降低 AChE 和 BChE 的活性以及提高 TDMF 的抗氧化状态可能是（）和（）表现出抗 AD 特性的部分机制。然而，（）比（）表现出更好的神经保护作用。
