Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China.
Health Management Center, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China.
Microbiol Spectr. 2022 Jun 29;10(3):e0055522. doi: 10.1128/spectrum.00555-22. Epub 2022 May 12.
KPC-24, different from KPC-2 by a single amino acid alteration at codon 6 (R6P), was initially discovered in Klebsiella pneumoniae in Chile. Here, we reported KPC-24-producing Aeromonas veronii isolates from hospital sewage in China. The was cloned and the MICs were tested against β-lactams antimicrobial agents. KPC-24 exhibited a β-lactam susceptibility profile similar to that of KPC-2. Whole-genome sequencing and analysis revealed that was located within a Tn-related region on an IncP-6 plasmid. Our study described a variant of K. pneumoniae carbapenemase (KPC), KPC-24, from two A. veronii strains isolated from hospital sewage, in which antibiotics, biocides, pharmaceuticals, and heavy metals may supply an appropriate condition for the evolution of carbapenemases. Some variants exhibited stronger hydrolysis activity to antibiotics and gave rise to a major public health concern. More seriously, species are prevalent in aquatic environments and, thus, may act as a suitable vector for antibiotics-resistance genes and foster the transmission of resistance. We should attach importance to surveying the evolution and transmission of antibiotics-resistance genes.
KPC-24 与 KPC-2 仅有一个氨基酸差异（第 6 位密码子的 R6P），最初在智利的肺炎克雷伯菌中发现。在此，我们报道了来自中国医院污水的产 KPC-24 气单胞菌分离株。我们克隆了并测试了对β-内酰胺类抗菌药物的 MIC。KPC-24 表现出与 KPC-2 相似的β-内酰胺类药物敏感性谱。全基因组测序和分析表明，位于 IncP-6 质粒上的一个 Tn 相关区域内。我们的研究描述了一种来自医院污水中分离的两种气单胞菌的肺炎克雷伯菌碳青霉烯酶（KPC）的变体 KPC-24，其中抗生素、消毒剂、药物和重金属可能为碳青霉烯酶的进化提供了合适的条件。一些变体对抗生素具有更强的水解活性，引起了主要的公共卫生关注。更严重的是，有些 种在水生环境中很普遍，因此可能成为抗生素耐药基因的合适载体，并促进耐药性的传播。我们应该重视调查抗生素耐药基因的进化和传播。
