Department of Oral Biology, Surgery and Biomedical Engineering, University at Buffalo, 3435 Main Street, B36A Foster Hall, Buffalo, NY, 14214, USA.
Oral Biology, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, 25000, Pakistan.
Sci Rep. 2022 May 11;12(1):7742. doi: 10.1038/s41598-022-11766-9.
Current biomaterials effectively replace biological structures but are limited by infections and long-term material failures. This study examined the molecular mechanisms of radio frequency glow discharge treatments (RFGDT) in mediating the disinfection of biomaterial surfaces and concurrently promoting cell attachment and proliferation. Dental biomaterials were subjected to RFGDT, and viability of oral microbial species, namely Streptococcus mutants (SM), Streptococcus gordonii (SG), Moraxella catarrhalis (MC), and Porphyromonas gingivalis (PG), were assessed. Cell attachment and survival of a pre-odontoblast cell line, MDPC-23, was examined. Finally, mechanistic investigations into redox generation and biological signaling were investigated. Based on their compositions, dental biomaterials induced reactive oxygen species (ROS) following dose-dependent RFGDT. Reduced microbial viability was evident following RFGDT in the catalase-negative (SM and SG) species more prominently than catalase-positive (MC and PG) species. Cell adhesion assays noted improved MDPC-23 attachment and survival. Pretreatments with N-acetylcysteine (NAC) and catalase abrogated these responses. Immunoassays noted redox-induced downstream expression of a laminin receptor, Ribosomal Protein SA, following RFGDT. Thus, RFGDT-induced redox mediates antimicrobial and improves cell responses such as adhesion and proliferation. These observations together provide a mechanistic rationale for the clinical utility of RFGDT with dental biomaterials for regenerative clinical applications.
目前的生物材料可以有效地替代生物结构，但它们受到感染和长期材料失效的限制。本研究探讨了射频辉光放电处理（RFGDT）在介导生物材料表面消毒的同时促进细胞附着和增殖的分子机制。对牙科生物材料进行了 RFGDT，评估了口腔微生物物种（即变形链球菌（SM）、戈登链球菌（SG）、卡他莫拉菌（MC）和牙龈卟啉单胞菌（PG））的活力。研究了预成牙本质细胞系 MDPC-23 的细胞附着和存活情况。最后，还研究了氧化还原生成和生物信号转导的机制。根据其组成，牙科生物材料在剂量依赖性 RFGDT 后会诱导产生活性氧（ROS）。RFGDT 后，CAT 阴性（SM 和 SG）物种的微生物活力明显降低，CAT 阳性（MC 和 PG）物种则不然。细胞附着实验表明 MDPC-23 的附着和存活得到改善。用 N-乙酰半胱氨酸（NAC）和 CAT 预处理可消除这些反应。免疫测定表明，RFGDT 后会引发层粘连蛋白受体和核糖体蛋白 SA 的下游表达。因此，RFGDT 诱导的氧化还原作用可以起到抗菌作用，并改善细胞的附着和增殖等反应。这些观察结果为 RFGDT 与牙科生物材料在再生临床应用中的临床应用提供了机制上的依据。
