Chen Yanru, Yan Zhangzhe, Su Yanping, Zhao Shang
Department of Histology & Embryology, Shandong First Medical University and Shandong Academy of Medical Sciences, Yingshengdong Road, Taishan District, Tai'an, Shandong China.
Department of Gastroenterology, Cancer Hospital Affiliated to Shandong First Medical University, Jiyan Road, Jinan City, Shandong, China.
J Coll Physicians Surg Pak. 2022 May;32(5):632-638. doi: 10.29271/jcpsp.2022.05.632.
Clear cell renal carcinoma (CCRC) is the most common type of renal carcinoma. We hope to find out the potential key genes playing important roles in CCRC genesis and progression by analysing the recent expression profiling by array from 2014 to 2016. In order to find out the differentially expressed genes (DEGs) between CCRC and normal renal tissue. Gene Ontology (GO) and Kyoto Encyclopedia of Genes, and Genomes (KEGG) pathway enrichment were carried out by the Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.8. Protein-Protein Interaction (PPI) Networks Functional Enrichment Analysis of these DEGs was analyzed using the Search Tool for Recurring Instances of Neighbouring Genes (STRING). The results were then visualized by the software Cytoscape. The authors also used the online tool of Kaplan-Meier plotter survival analysis to assess the significance of the top ten genes in the prognosis of CCRC. A total of 192 DEGs were identified and the top ten key genes were picked out by the software Cytoscape. FN1, CXCR4, LOX, and PLG were then further screened out based on the overall survival analysis; SLC12A1 and LOX were screened out after the recurrence-free survival analysis. LOX was finally believed to be the most reliable prognostic factor since it has prognostic value for both overall survival and recurrence-free survival analysis. Our analysis suggests that LOX is the most reliable prognostic factor for CCRC patients. Key Words: Renal clear cell carcinoma, Microarray datasets, Bioinformatics approach, Prognostic factor, LOX.
透明细胞肾细胞癌（CCRC）是最常见的肾癌类型。我们希望通过分析2014年至2016年最近的基因芯片表达谱，找出在CCRC发生和发展中起重要作用的潜在关键基因。为了找出CCRC与正常肾组织之间的差异表达基因（DEG）。通过注释、可视化和综合发现数据库（DAVID）v6.8进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路富集分析。使用相邻基因重复实例搜索工具（STRING）对这些DEG进行蛋白质-蛋白质相互作用（PPI）网络功能富集分析。然后通过Cytoscape软件对结果进行可视化。作者还使用Kaplan-Meier绘图仪生存分析在线工具评估前十个基因在CCRC预后中的意义。共鉴定出192个DEG，并通过Cytoscape软件挑选出前十个关键基因。然后根据总生存分析进一步筛选出纤连蛋白1（FN1）、趋化因子受体4（CXCR4）、赖氨氧化酶（LOX）和纤溶酶原（PLG）；无复发生存分析后筛选出溶质载体家族12成员1（SLC12A1）和LOX。最终认为LOX是最可靠的预后因素，因为它对总生存和无复发生存分析均具有预后价值。我们的分析表明，LOX是CCRC患者最可靠的预后因素。关键词：肾透明细胞癌；基因芯片数据集；生物信息学方法；预后因素；LOX
