Kang Jiefang, Guo Chen, Thome Rodolfo, Yang Ning, Zhang Yuan, Li Xing, Cao Xiaoyan
Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University Xi'an Shaanxi China
Department of Neurology, Thomas Jefferson University Philadelphia PA 19107 USA
RSC Adv. 2018 Aug 29;8(53):30539-30549. doi: 10.1039/c8ra06045b. eCollection 2018 Aug 24.
Iridoid glycosides (CIG) are the major component of . In this work, we researched the antioxidative, hypoglycemic and lowering blood lipids effects of CIG on diabetic mice induced by a high-fat diet (HFD) and streptozotocin (STZ). Furthermore, to investigate the molecular mechanism of action, the phosphorylation and protein expression of phosphoinositide 3-kinase (PI3K) and its downstream proteins, such as insulin receptor (INSR), protein kinase B (Akt/PKB) and glucose transporter 4 (GLUT4) have been detected. The results showed that CIG significantly improved oral glucose tolerance in diabetic mice. Biochemical indices also revealed that CIG had a positive effect on lipid metabolism and oxidative stress. In addition, CIG can significantly enhance the expression level of the PI3K-Akt/PKB pathway related proteins in skeletal muscle, which is the key pathway of insulin metabolism. These findings show that CIG can improve the hyperglycemia and hyperlipidemia of HFD-STZ-induced diabetic mice through the PI3K-Akt/PKB signaling pathway, and CIG might be a potential medicine or functional food for type 2 diabetes mellitus remedies.
环烯醚萜苷（CIG）是[具体物质]的主要成分。在本研究中，我们研究了CIG对高脂饮食（HFD）和链脲佐菌素（STZ）诱导的糖尿病小鼠的抗氧化、降血糖和降血脂作用。此外，为了探究其分子作用机制，检测了磷酸肌醇3激酶（PI3K）及其下游蛋白，如胰岛素受体（INSR）、蛋白激酶B（Akt/PKB）和葡萄糖转运蛋白4（GLUT4）的磷酸化和蛋白表达。结果表明，CIG显著改善了糖尿病小鼠的口服葡萄糖耐量。生化指标还显示，CIG对脂质代谢和氧化应激有积极作用。此外，CIG可显著提高骨骼肌中PI3K-Akt/PKB信号通路相关蛋白的表达水平，这是胰岛素代谢的关键通路。这些发现表明，CIG可通过PI3K-Akt/PKB信号通路改善HFD-STZ诱导的糖尿病小鼠的高血糖和高血脂，CIG可能是治疗2型糖尿病的潜在药物或功能性食品。
