He Yuan, Shi Yang, Zhang Airong, Zhang Xiaoxia, Sun Jing, Tian Li
College of Traditional Chinese Medicine, Xinjiang Medical University Urumqi Xinjiang China
Department of Editor, Xinjiang Medical University Urumqi Xinjiang China.
RSC Adv. 2019 Aug 27;9(46):26757-26767. doi: 10.1039/c9ra04481g. eCollection 2019 Aug 23.
Flavonoids are the major components of L. root (AR). In this study, we used a rat model of high-fat emulsion die induced hyperlipidemia (HLP) to examine the antioxidant and lipid-lowering effects of the AR flavonoid extract (ARFE), and to identify its potential targets by molecular docking and western-blotting. ARFE significantly lowered the serum lipid levels in HLP rats. The lipid-lowering targets of ARFE were predicted and validated, which showed that ARFE significantly downregulated the expression levels of cholesterol synthesis-associated target proteins, including SREBP1, HMGCR and ACC1. These findings demonstrate that ARFE has remarkable lipid-lowering and antioxidant effects, and the former is mediated by the inhibition of SREBP1, HMGCR and ACC1.
黄酮类化合物是L.根（AR）的主要成分。在本研究中，我们使用高脂乳剂诱导的高脂血症（HLP）大鼠模型来研究AR黄酮提取物（ARFE）的抗氧化和降脂作用，并通过分子对接和蛋白质免疫印迹法确定其潜在靶点。ARFE显著降低了HLP大鼠的血脂水平。预测并验证了ARFE的降脂靶点，结果表明ARFE显著下调了胆固醇合成相关靶蛋白的表达水平，包括SREBP1、HMGCR和ACC1。这些发现表明，ARFE具有显著的降脂和抗氧化作用，前者是通过抑制SREBP1、HMGCR和ACC1介导的。
