一种利用CYR稳定的G-四链体形成对生乳中噻虫嗪进行检测的简单荧光分析法。

A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation.

作者信息

Xing Haibo, Gu Wenchao, Xu Dang, Tian Fuxiang, Yao Linyun, Wang Zhenwei, Hu Xiaojun

机构信息

School of Chemical and Environmental Engineering, Shanghai Institute of Technology Shanghai 201418 China

Putuo District Center for Disease Control and Prevention Shanghai China.

出版信息

RSC Adv. 2018 Jan 10;8(5):2418-2425. doi: 10.1039/c7ra12970j. eCollection 2018 Jan 9.

A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result. When an FAM labelled G-rich ssDNA Tcy2 is treated with cyromazine, it can form a G-quadruplex-CYR complex and cause a change in fluorescence. As a result, the presence of cyromazine can be determined by fluorescence quenching. This sensor is selective for the detection of cyromazine in raw milk and has a limit of detection of 0.68 ppb and a detection range from 0 to 200 ppb.