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一种利用CYR稳定的G-四链体形成对生乳中噻虫嗪进行检测的简单荧光分析法。

A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation.

作者信息

Xing Haibo, Gu Wenchao, Xu Dang, Tian Fuxiang, Yao Linyun, Wang Zhenwei, Hu Xiaojun

机构信息

School of Chemical and Environmental Engineering, Shanghai Institute of Technology Shanghai 201418 China

Putuo District Center for Disease Control and Prevention Shanghai China.

出版信息

RSC Adv. 2018 Jan 10;8(5):2418-2425. doi: 10.1039/c7ra12970j. eCollection 2018 Jan 9.

DOI:10.1039/c7ra12970j
PMID:35546964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9088062/
Abstract

A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result. When an FAM labelled G-rich ssDNA Tcy2 is treated with cyromazine, it can form a G-quadruplex-CYR complex and cause a change in fluorescence. As a result, the presence of cyromazine can be determined by fluorescence quenching. This sensor is selective for the detection of cyromazine in raw milk and has a limit of detection of 0.68 ppb and a detection range from 0 to 200 ppb.

摘要

基于荧光猝灭结果报道了一种用于检测牛奶中环丙氨嗪的快速生物传感器。当用环丙氨嗪处理荧光素标记的富含鸟嘌呤的单链DNA Tcy2时，它可以形成G-四链体-CYR复合物并导致荧光变化。因此，环丙氨嗪的存在可以通过荧光猝灭来确定。该传感器对生牛奶中环丙氨嗪的检测具有选择性，检测限为0.68 ppb，检测范围为0至200 ppb。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/885e/9088062/dc426f8e05ab/c7ra12970j-f7.jpg
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