Fan Huanhuan, Yan Guobei, Zhao Zilong, Hu Xiaoxiao, Zhang Wenhan, Liu Hui, Fu Xiaoyi, Fu Ting, Zhang Xiao-Bing, Tan Weihong
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082, China.
Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute, McKnight Brain Institute, University of Florida, Gainesville, FL, 32611-7200, USA.
Angew Chem Int Ed Engl. 2016 Apr 25;55(18):5477-82. doi: 10.1002/anie.201510748. Epub 2016 Mar 24.
Photodynamic therapy (PDT) has been applied in cancer treatment by utilizing reactive oxygen species to kill cancer cells. However, a high concentration of glutathione (GSH) is present in cancer cells and can consume reactive oxygen species. To address this problem, we report the development of a photosensitizer-MnO2 nanosystem for highly efficient PDT. In our design, MnO2 nanosheets adsorb photosensitizer chlorin e6 (Ce6), protect it from self-destruction upon light irradiation, and efficiently deliver it into cells. The nanosystem also inhibits extracellular singlet oxygen generation by Ce6, leading to fewer side effects. Once endocytosed, the MnO2 nanosheets are reduced by intracellular GSH. As a result, the nanosystem is disintegrated, simultaneously releasing Ce6 and decreasing the level of GSH for highly efficient PDT. Moreover, fluorescence recovery, accompanied by the dissolution of MnO2 nanosheets, can provide a fluorescence signal for monitoring the efficacy of delivery.
光动力疗法（PDT）已通过利用活性氧来杀死癌细胞而应用于癌症治疗。然而，癌细胞中存在高浓度的谷胱甘肽（GSH），其能够消耗活性氧。为了解决这一问题，我们报道了一种用于高效光动力疗法的光敏剂 - 二氧化锰纳米系统的研发。在我们的设计中，二氧化锰纳米片吸附光敏剂二氢卟吩e6（Ce6），保护其在光照下不发生自我破坏，并有效地将其递送至细胞内。该纳米系统还能抑制Ce6在细胞外产生单线态氧，从而减少副作用。一旦被内吞，二氧化锰纳米片会被细胞内的GSH还原。结果，该纳米系统解体，同时释放Ce6并降低GSH水平以实现高效光动力疗法。此外，伴随二氧化锰纳米片溶解的荧光恢复可为监测递送效果提供荧光信号。
