Paul Saptadipa, Majumdar Mala
Department of Biotechnology, School of Sciences, JAIN (Deemed to be University), #34,1st Cross, J C Road, Bangalore, 560027 India.
3 Biotech. 2022 May;12(5):114. doi: 10.1007/s13205-022-03166-7. Epub 2022 Apr 17.
Commercial antidiabetic polyherbal formulations (APH) are available with claimed hypoglycemic activities; yet they lack systematic scientific studies leading to their limited global acceptance. In the present study, six selected APH from the Indian market were evaluated for their phytochemical contents, anti-hyperglycemic, anti-hyperlipidemic, antioxidant activities and further identifying the major antidiabetic bioactive compound of "MA" by HPLC-ESI-MS/MS. Our results revealed highest TPC (136.97 ± 0.6 µg GAE/mg) and TFC (128.85 ± 0.74 µg QE/mg) in APH-DB and APH-SN, respectively. APH-MA has exhibited highest α-amylase 72.5% (IC-579.65 μg/ml), α-glucosidase 88.02% (IC-261.03 μg/ml) and moderate lipase inhibition 57.7% (IC 159.57 μg/ml). A variable free radical scavenging activity was observed by all the tested APH. Further significant linear positive correlations were observed between TPC-Lipase ( -0.985****), TFC-α-amylase ( -0.868**) and DPPH-α-amylase inhibition ( -0.8098*). HPLC-ESI-MS/MS of MA showed the presence of anti-hyperglycemic compounds, Pheophorbide a and Pyropheophorbide a, as the major peaks. Among the tested extracts, MA exhibited better activities while BG, MH, SN, DB, and DT have showed comparable/mild anti-hyperglycemic, anti-hyperlipidemic and antioxidant potential. Hence the tested APH may be considered effective for DM management which can further be assessed for their other targets of inhibition.
市面上有宣称具有降血糖活性的商业抗糖尿病多草药配方（APH）；然而，它们缺乏系统的科学研究，导致其在全球的接受度有限。在本研究中，对从印度市场挑选的六种APH进行了植物化学成分、抗高血糖、抗高血脂、抗氧化活性评估，并通过HPLC-ESI-MS/MS进一步鉴定了“MA”的主要抗糖尿病生物活性化合物。我们的结果显示，APH-DB和APH-SN中的总酚含量（TPC）分别最高（136.97±0.6μg没食子酸当量/mg）和总黄酮含量（TFC）最高（128.85±0.74μg芦丁当量/mg）。APH-MA对α-淀粉酶的抑制率最高达72.5%（IC50为79.65μg/ml），对α-葡萄糖苷酶的抑制率为88.02%（IC50为261.03μg/ml），对脂肪酶的抑制率为中等水平，为57.7%（IC50为159.57μg/ml）。所有测试的APH均表现出不同程度的自由基清除活性。此外，还观察到总酚含量与脂肪酶抑制率之间存在显著的线性正相关（-0.985****）、总黄酮含量与α-淀粉酶抑制率之间存在显著的线性正相关（-0.868**）以及DPPH与α-淀粉酶抑制率之间存在显著的线性正相关（-0.8098*）。MA的HPLC-ESI-MS/MS分析表明，主要峰中存在抗高血糖化合物脱镁叶绿酸a和焦脱镁叶绿酸a。在测试的提取物中，MA表现出更好的活性，而BG、MH、SN、DB和DT则表现出相当/温和的抗高血糖、抗高血脂和抗氧化潜力。因此，测试的APH可被认为对糖尿病管理有效，可进一步评估其其他抑制靶点。
