Unuofin Jeremiah Oshiomame, Otunola Gloria Aderonke, Afolayan Anthony Jide
Medicinal Plants and Economic Development (MPED) Research Centre, Department of Botany, University of Fort Hare, Alice 5700, South Africa.
Heliyon. 2018 Sep 27;4(9):e00810. doi: 10.1016/j.heliyon.2018.e00810. eCollection 2018 Sep.
, is a tuberous plant commonly used by traditional healers in the Eastern Cape Province of South Africa for the management of obesity. The aim of this study was to investigate the antiobesity and cytotoxic effects of extracts in vitro The α-amylase, α-glucosidase and lipase inhibitory activities of aqueous and ethanol extracts of tuber were investigated while the cytotoxic effects of these extracts were analyzed using Hoechst 33342 and propidium iodide (PI) dual staining in combination with Molecular Devices ImageXpress Micro XLS Widefield microscope for high content analysis on human cervical (HeLa) cell line. The ethanol extract exhibited the strongest inhibitory effect on pancreatic lipase (IC = 381.86 μg/ml) and on α-glucosidase (IC = 157.99 μg/mL) while the aqueous extract has strongest α-amylase (IC = 439.45 μg/ml). Both tuber extracts were found nontoxic at tested concentrations on HeLa cell lines as confirmed by the Hoechst 33342 and propidium iodide dual staining respectively. This study revealed that both the aqueous and ethanol tuber extract of exerts a certain degree of inhibitory effect on α-amylase, α-glucosidase and lipase and were also nontoxic to HeLa cell line at tested concentrations.
是一种块茎植物，南非东开普省的传统治疗师常用它来治疗肥胖症。本研究的目的是体外研究提取物的抗肥胖和细胞毒性作用。研究了块茎的水提取物和乙醇提取物的α-淀粉酶、α-葡萄糖苷酶和脂肪酶抑制活性，同时使用Hoechst 33342和碘化丙啶（PI）双重染色结合Molecular Devices ImageXpress Micro XLS Widefield显微镜对人宫颈（HeLa）细胞系进行高内涵分析，以分析这些提取物的细胞毒性作用。乙醇提取物对胰腺脂肪酶（IC = 381.86 μg/ml）和α-葡萄糖苷酶（IC = 157.99 μg/mL）表现出最强的抑制作用，而水提取物对α-淀粉酶（IC = 439.45 μg/ml）具有最强的抑制作用。通过Hoechst 33342和碘化丙啶双重染色分别证实，两种块茎提取物在测试浓度下对HeLa细胞系均无毒。本研究表明，块茎的水提取物和乙醇提取物对α-淀粉酶、α-葡萄糖苷酶和脂肪酶均有一定程度的抑制作用，并且在测试浓度下对HeLa细胞系也无毒。
