Wang Xiaoxu, Liu Jieying, Hui Xiangyi, Song Yingna
Department of Obstetrics and Gynecology, National Clinical Research Centre for Obstetric and Gynecologic Diseases, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
State Key Laboratory of Complex Severe and Rare Diseases, Medical Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Front Pediatr. 2022 Apr 25;10:869381. doi: 10.3389/fped.2022.869381. eCollection 2022.
Preeclampsia (PE) is one of the leading causes of maternal and perinatal morbidity and mortality. However, it is still uncertain how PE affects neonate metabolism. We conducted an untargeted metabolomics analysis of cord blood to explore the metabolic changes in PE neonates. Umbilical cord serum samples from neonates with preeclampsia ( = 29) and non-preeclampsia (non-PE) ( = 32) pregnancies were analyzed using the UHPLC-QE-MS metabolomic platform. Different metabolites were screened, and pathway analysis was conducted. A subgroup analysis was performed among PE neonates to compare the metabolome between appropriate-for-gestational-age infants ( = 21) and small-for-gestational-age (SGA) infants ( = 8). A total of 159 different metabolites were detected in PE and non-PE neonates. Creatinine, N4-acetylcytidine, sphingomyelin (D18:1/16:0), pseudouridine, uric acid, and indolelactic acid were the most significant differential metabolites in the cord serum of PE neonates. Differential metabolite levels were elevated in PE neonates and were involved in the following metabolic pathways: glycine, serine, and threonine metabolism; sphingolipid, glyoxylate, and dicarboxylate metabolism; and arginine biosynthesis. In PE neonates, SGA neonates showed increased levels of hexacosanoyl carnitine and decreased abundance of 3-hydroxybutyric acid and 3-sulfinoalanine. Taurine-related metabolism and ketone body-related pathways were mainly affected. Based on the UHPLC-QE-MS metabolomics analysis, we identified the metabolic profiles of PE and SGA neonates. The abundance of metabolites related to certain amino acid, sphingolipid, and energy metabolism increased in the umbilical cord serum of PE neonates.
子痫前期（PE）是孕产妇和围产儿发病及死亡的主要原因之一。然而，PE如何影响新生儿代谢仍不确定。我们对脐带血进行了非靶向代谢组学分析，以探索PE新生儿的代谢变化。使用超高效液相色谱-四极杆飞行时间质谱（UHPLC-QE-MS）代谢组学平台分析了子痫前期（n = 29）和非子痫前期（非PE）（n = 32）妊娠新生儿的脐带血清样本。筛选出不同的代谢物，并进行通路分析。在PE新生儿中进行亚组分析，以比较适于胎龄儿（n = 21）和小于胎龄儿（SGA）（n = 8）之间的代谢组。在PE和非PE新生儿中总共检测到159种不同的代谢物。肌酐、N4-乙酰胞苷、鞘磷脂（D18:1/16:0）、假尿苷、尿酸和吲哚乳酸是PE新生儿脐带血清中最显著的差异代谢物。PE新生儿中差异代谢物水平升高，并参与以下代谢途径：甘氨酸、丝氨酸和苏氨酸代谢；鞘脂、乙醛酸和二羧酸代谢；以及精氨酸生物合成。在PE新生儿中，SGA新生儿的二十六烷酰肉碱水平升高，3-羟基丁酸和3-亚磺基丙氨酸丰度降低。牛磺酸相关代谢和酮体相关途径主要受到影响。基于UHPLC-QE-MS代谢组学分析，我们确定了PE和SGA新生儿的代谢谱。PE新生儿脐带血清中与某些氨基酸、鞘脂和能量代谢相关的代谢物丰度增加。
