Straub K M, Rudewicz P, Garvie C
Xenobiotica. 1987 Mar;17(3):413-22. doi: 10.3109/00498258709043948.
The development of tandem mass spectrometry (m.s./m.s.) techniques for the rapid structural elucidation and quantification of metabolites is described. With these techniques the mass spectrometer serves as both a separation device as well as a tool for structure elucidation. Ion formation by desorption ionization techniques and mass analysis with an appropriate combination of neutral loss and precursor ion scans can then be used to classify the number and type of primary metabolites and polar drug conjugates that are present in a complex sample matrix. The resulting information can be used to assess the overall biotransformation routes that are available to a drug or other xenobiotic substance.
本文描述了串联质谱（m.s./m.s.）技术在代谢物快速结构解析和定量分析方面的发展。利用这些技术，质谱仪既作为一种分离装置，又作为结构解析的工具。通过解吸电离技术形成离子，并结合中性丢失和前体离子扫描进行质量分析，可用于对复杂样品基质中存在的主要代谢物和极性药物共轭物的数量和类型进行分类。所得信息可用于评估药物或其他外源性物质可用的整体生物转化途径。
