Liu Jie, Yin Pinghe, Zhao Ling
Department of Chemistry, Jinan University Guangzhou 510632 People's Republic of China.
Research Center of Analytical Testing, Jinan University Guangzhou 510632 China
RSC Adv. 2018 Aug 16;8(51):29172-29178. doi: 10.1039/c8ra05156a. eCollection 2018 Aug 14.
Macroalgae, the major contributor of primary productivity in coastal seas, contribute to the material cycle and energy flow in marine ecosystems. The purpose of this work was to evaluate the toxic effect of nano-TiO on the growth and antioxidant activity of . An obvious inhibition of growth was observed in this study. The algae exposed to nano-TiO showed a negative growth rate at 20 mg L and 40 mg L during the 15 days exposure. The concentration of soluble protein increased slightly during the first 3 days of exposure, but it gradually diminished thereafter due to the high concentrations of nano-TiO and to prolonged exposure. Nano-TiO caused oxidative damage in ; superoxide anions accumulated, and nitrate reductase activity decreased linearly with the increase in nano-TiO. Furthermore, extracts of can scavenge DPPH· and hydroxyl radicals for their antioxidant capacity. However, the capacity to scavenge DPPH· and hydroxyl radicals decreased slightly with the increase in nano-TiO. The results from this work imply that macroalgae can be an effective biomarker of nano-TiO contamination and can be useful indicators to evaluate the oxidative damage of increasing pollutants in marine ecosystems.
大型海藻是沿海水域初级生产力的主要贡献者，对海洋生态系统中的物质循环和能量流动有重要作用。本研究的目的是评估纳米二氧化钛对[大型海藻名称未给出]生长和抗氧化活性的毒性影响。本研究观察到明显的生长抑制现象。在15天的暴露期内，暴露于纳米二氧化钛的大型海藻在20毫克/升和40毫克/升浓度下呈现负生长率。暴露的前3天，可溶性蛋白浓度略有增加，但由于纳米二氧化钛浓度过高和暴露时间延长，此后逐渐下降。纳米二氧化钛对[大型海藻名称未给出]造成氧化损伤；超氧阴离子积累，硝酸还原酶活性随纳米二氧化钛浓度增加呈线性下降。此外，[大型海藻名称未给出]提取物因其抗氧化能力可清除二苯基苦味酰基自由基（DPPH·）和羟基自由基。然而，清除DPPH·和羟基自由基的能力随纳米二氧化钛浓度增加略有下降。本研究结果表明，大型海藻可以作为纳米二氧化钛污染的有效生物标志物，也是评估海洋生态系统中污染物增加所导致氧化损伤的有用指标。
