Li Ruiqiong, Zou Jianan, Sun Dongming, Jing Yan, Wu Depeng, Lian Ming, Teng Weili, Zhan Yuhang, Li Wenbin, Zhao Xue, Han Yingpeng
Key Laboratory of Soybean Biology in Chinese Ministry of Education (Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry), Northeast Agricultural University, Harbin, China.
College of Tropical Crops, Hainan University, Haikou, China.
Front Plant Sci. 2022 Apr 25;13:865584. doi: 10.3389/fpls.2022.865584. eCollection 2022.
Isoflavones, one of the most important secondary metabolites produced by soybeans ( (L.) Merr.), are important for a variety of biological processes, and are beneficial for human health. To identify genetic loci underlying soybean isoflavone content, a mapping population containing 119 F recombinant inbred lines, derived by crossing soybean cultivar "Zhongdou27" with "Dongong8004," was used. We identified 15 QTLs associated with isoflavone contents. A novel loci, qISO19-1, was mapped onto soybean chromosome 19 and was fine-mapped to a 62.8 kb region using a BCF population. We considered as a candidate gene for the qISO19-1 locus due to the significant positive correlation recovered between its expression level and isoflavone content in the seeds of 43 soybean germplasms. Overexpression of in and soybean cultivars increased isoflavone contents. Transgenic soybeans overexpressing also exhibited improved resistance to pathogenic infection, while transgenic resisted salt and drought stress.
异黄酮是大豆（Glycine max (L.) Merr.）产生的最重要的次生代谢产物之一，对多种生物学过程都很重要，且对人体健康有益。为了鉴定大豆异黄酮含量的遗传位点，使用了一个由大豆品种“中豆27”与“东农8004”杂交衍生的包含119个F重组自交系的作图群体。我们鉴定出了15个与异黄酮含量相关的QTL。一个新的位点qISO19 - 1被定位到大豆第19号染色体上，并使用一个BCF群体将其精细定位到一个62.8 kb的区域。由于在43份大豆种质的种子中其表达水平与异黄酮含量之间存在显著正相关，我们认为 是qISO19 - 1位点的候选基因。 在拟南芥和大豆品种中的过表达增加了异黄酮含量。过表达 的转基因大豆对病原体感染的抗性也有所提高，而过表达 的转基因大豆则能抵抗盐和干旱胁迫。
