Yang Rui, Li Murong, Harrison Matthew Tom, Fahad Shah, Wei Mingmei, Li Xiu, Yin Lijun, Sha Aihua, Zhou Meixue, Liu Ke, Wang Xiaoyan
Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, China.
Agriculture College, Yangtze University, Jingzhou, China.
Front Plant Sci. 2022 Apr 25;13:890083. doi: 10.3389/fpls.2022.890083. eCollection 2022.
Transient and chronic waterlogging constrains crop production in many regions of the world. Here, we invoke a novel iTRAQ-based proteomic strategy to elicit protein synthesis and regulation responses to waterlogging in tolerant (XM 55) and sensitive genotypes (YM 158). Of the 7,710 proteins identified, 16 were distinct between the two genotypes under waterlogging, partially defining a proteomic basis for waterlogging tolerance (and sensitivity). We found that 11 proteins were up-regulated and 5 proteins were down-regulated; the former included an Fe-S cluster assembly factor, heat shock cognate 70, GTP-binding protein SAR1A-like and CBS domain-containing protein. Down-regulated proteins contained photosystem II reaction center protein H, carotenoid 9, 10 (9', 10')-cleavage dioxygenase-like, psbP-like protein 1 and mitochondrial ATPase inhibitor. We showed that nine proteins responded to waterlogging with non-cultivar specificity: these included 3-isopropylmalate dehydratase large subunit, solanesyl-diphosphate synthase 2, DEAD-box ATP-dependent RNA helicase 3, and 3 predicted or uncharacterized proteins. Sixteen of the 28 selected proteins showed consistent expression patterns between mRNA and protein levels. We conclude that waterlogging stress may redirect protein synthesis, reduce chlorophyll synthesis and enzyme abundance involved in photorespiration, thus influencing synthesis of other metabolic enzymes. Collectively, these factors accelerate the accumulation of harmful metabolites in leaves in waterlogging-susceptible genotypes. The differentially expressed proteins enumerated here could be used as biological markers for enhancing waterlogging tolerance as part of future crop breeding programs.
短期和长期渍水限制了世界上许多地区的作物产量。在此，我们采用了一种基于iTRAQ的新型蛋白质组学策略，以探究耐渍（XM 55）和敏感基因型（YM 158）对渍水的蛋白质合成及调控反应。在鉴定出的7710种蛋白质中，有16种在渍水条件下的两种基因型之间存在差异，部分确定了渍水耐受性（和敏感性）的蛋白质组学基础。我们发现有11种蛋白质上调，5种蛋白质下调；前者包括一个铁硫簇组装因子、热休克同源蛋白70、类GTP结合蛋白SAR1A和含CBS结构域的蛋白质。下调的蛋白质包括光系统II反应中心蛋白H、类胡萝卜素9,10(9',10')-裂解双加氧酶、类psbP蛋白1和线粒体ATP酶抑制剂。我们表明，有9种蛋白质对渍水的反应不具有品种特异性：其中包括3-异丙基苹果酸脱水酶大亚基、茄尼基二磷酸合酶2、依赖ATP的DEAD盒RNA解旋酶3以及3种预测或未鉴定的蛋白质。在所选的28种蛋白质中，有16种在mRNA和蛋白质水平之间表现出一致的表达模式。我们得出结论，渍水胁迫可能会改变蛋白质合成方向，减少参与光呼吸的叶绿素合成和酶丰度，从而影响其他代谢酶的合成。总的来说，这些因素加速了渍水敏感基因型叶片中有害代谢物的积累。这里列举的差异表达蛋白质可作为生物标志物，用于在未来的作物育种计划中提高渍水耐受性。
