Azarkina Regina, Makeeva Arina, Mamaeva Anna, Kovalchuk Sergey, Ganaeva Daria, Tikhonovich Igor, Fesenko Igor
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow 117997, Russia.
Laboratory of System Analysis of Proteins and Peptides, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Plants (Basel). 2025 Jul 14;14(14):2168. doi: 10.3390/plants14142168.
Drought conditions impact plants at the morphological, physiological, and molecular levels. Plant tolerance to drought conditions is frequently associated with maintaining proteome stability, highlighting the significance of proteomic analysis in understanding the mechanisms underlying plant resilience. Here, we performed proteomic and peptidomic analysis of spring wheat ( L.) under drought stress conditions. Using isobaric tags for relative and absolute quantitation (iTRAQ), we identified 497 and 157 differentially abundant protein (DAP) groups in leaves and roots, respectively. The upregulated DAP groups in leaves were primarily involved in stress responses, such as oxidative stress and heat response, whereas those in roots were associated with responses to water deprivation and sulfur compound metabolic processes. The analysis of the extracellular root peptidome revealed 2294 native peptides, including members of small secreted peptide (SSP) families. In the peptidomes of stress-induced plants, we identified 16 SSPs as well as peptides derived from proteins involved in cell wall catabolism, intercellular signaling, and stress response. These peptides represent potential candidates as regulators of drought responses. Our results help us to understand adaptation mechanisms and develop new agricultural technologies to increase productivity.
干旱条件会在形态、生理和分子水平上影响植物。植物对干旱条件的耐受性通常与维持蛋白质组稳定性相关，这凸显了蛋白质组学分析在理解植物抗逆机制方面的重要性。在此，我们对干旱胁迫条件下的春小麦（L.）进行了蛋白质组学和肽组学分析。使用等压标签相对和绝对定量（iTRAQ）技术，我们分别在叶片和根中鉴定出497个和157个差异丰富蛋白质（DAP）组。叶片中上调的DAP组主要参与应激反应，如氧化应激和热反应，而根中的DAP组则与缺水反应和硫化合物代谢过程相关。对根细胞外肽组的分析揭示了2294种天然肽，包括小分泌肽（SSP）家族的成员。在胁迫诱导植物的肽组中，我们鉴定出16种SSP以及来自参与细胞壁分解代谢、细胞间信号传导和应激反应的蛋白质的肽。这些肽代表了作为干旱反应调节因子的潜在候选物。我们的结果有助于我们理解适应机制并开发新的农业技术以提高生产力。
