Kang Moon Sung, Kang Jeon Il, Le Thi Phuong, Park Kyung Min, Hong Suck Won, Choi Yu Suk, Han Dong-Wook, Park Ki Dong
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46241, Republic of Korea.
Department of Bioengineering and Nano-bioengineering, Incheon National University, Incheon 22012, Republic of Korea.
ACS Macro Lett. 2021 Apr 20;10(4):426-432. doi: 10.1021/acsmacrolett.0c00845. Epub 2021 Apr 3.
Three-dimensional (3D) bioprinting has attracted considerable attention for producing 3D engineered cellular microenvironments that replicate complex and sophisticated native extracellular matrices (ECM) as well as the spatiotemporal gradients of numerous physicochemical and biological cues. Although various hydrogel-based bioinks have been reported, the development of advanced bioink materials that can reproduce the complexity of ECM accurately and mimic the intrinsic property of laden cells is still a challenge. This paper reports 3D printable bioinks composed of phenol-rich gelatin (GHPA) and graphene oxide (GO) as a component for a myogenesis-inducing material, which can form a hydrogel network in situ by a dual enzyme-mediated cross-linking reaction. The in situ curable GO/GHPA hydrogel can be utilized successfully as 3D-printable bioinks to provide suitable cellular microenvironments with facilitated myogenic differentiation of C2C12 skeletal myoblasts. Overall, we suggest that functional bioinks may be useful in muscle tissue engineering and regenerative medicine.
三维（3D）生物打印因能够制造出三维工程化细胞微环境而备受关注，这种微环境可复制复杂且精密的天然细胞外基质（ECM）以及众多物理化学和生物学信号的时空梯度。尽管已有各种基于水凝胶的生物墨水被报道，但开发能够精确再现ECM复杂性并模拟负载细胞固有特性的先进生物墨水材料仍是一项挑战。本文报道了由富含苯酚的明胶（GHPA）和氧化石墨烯（GO）组成的3D可打印生物墨水，作为一种诱导成肌的材料成分，其可通过双酶介导的交联反应原位形成水凝胶网络。原位可固化的GO/GHPA水凝胶能够成功用作3D可打印生物墨水，为C2C12骨骼肌成肌细胞的成肌分化提供合适的细胞微环境。总体而言，我们认为功能性生物墨水在肌肉组织工程和再生医学中可能会有用。
