Hussein Kamal H, Soliman Mahmoud, Sabra Mahmoud S, Abdelhamid Hani Nasser, Abd-Elkareem Mahmoud, Sadek Ahmed Abdelrahiem
Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71516, Egypt.
Tissue Culture and Stem Cells Unit, Molecular Biology Researches & Studies Institute, Assiut University, Assiut, 71526, Egypt.
BMC Vet Res. 2025 May 7;21(1):324. doi: 10.1186/s12917-025-04721-z.
Delayed wound closure and non-healing wounds represent a problematic condition with health burden and an economic challenge. Therefore, different strategies have been developed, including skin tissue engineering, which aims to stimulate and support the wound healing process. In this study, the potential of graphene oxide (GO) and chitosan (CTS) biomaterial composite, with and without fetal bovine serum (FBS), was investigated to induce a full-thickness skin wound repair in rats.
The GO-CTS composite was characterized using X-ray diffraction, transmission electron microscopy, and Fourier transforms infrared. Cytocompatibility was evaluated via an MTT assay with human endothelial cells (ECs) and mouse embryonic fibroblasts (MEFs) in vitro. The in vivo wound regeneration potential was assessed by creating an 8 mm full-thickness circular skin defect on the dorsal surface of the rat. The defects were randomly divided into control, GO-CTS, FBS, and GO-CTS/FBS groups, and were monitored grossly and histologically at days 7 and 21 after wound induction.
The GO-CTS material demonstrated high cytocompatibility, with cell viability recorded at 99.2% ± 5.7% for ECs and 110.5% ± 3.9% for MEFs. The highest proliferation rates were observed in the FBS (118.2% ± 2.1%) and GO-CTS/FBS (121.4% ± 4.4%) groups. In vivo, wound closure rates on day 21 were 85.5% ± 0.56% for GO-CTS, 87.5% ± 1.75% for FBS, and 91.5% ± 1.03% for GO-CTS/FBS, all significantly higher than the control group. Additionally, neovascularization, epithelialization, collagen deposition, and granulation tissue formation were more prominent in the treated groups, with skin appendages observed in the GO-CTS/FBS group.
GO-CTS nanosheets with FBS represent a promising biomaterial for skin tissue engineering and can effectively initiate and support wound healing.
伤口延迟愈合和不愈合是一种存在健康负担且带来经济挑战的问题状况。因此，人们开发了不同的策略，包括皮肤组织工程，其旨在刺激和支持伤口愈合过程。在本研究中，研究了含和不含胎牛血清（FBS）的氧化石墨烯（GO）和壳聚糖（CTS）生物材料复合材料诱导大鼠全层皮肤伤口修复的潜力。
使用X射线衍射、透射电子显微镜和傅里叶变换红外光谱对GO-CTS复合材料进行表征。通过MTT法在体外评估人内皮细胞（ECs）和小鼠胚胎成纤维细胞（MEFs）的细胞相容性。通过在大鼠背部表面制造一个8毫米的全层圆形皮肤缺损来评估体内伤口再生潜力。将缺损随机分为对照组、GO-CTS组、FBS组和GO-CTS/FBS组，并在伤口诱导后第7天和第21天进行大体和组织学监测。
GO-CTS材料表现出高细胞相容性，ECs的细胞活力记录为99.2%±5.7%，MEFs的细胞活力记录为110.5%±3.9%。在FBS组（118.2%±2.1%）和GO-CTS/FBS组（121.4%±4.4%）中观察到最高的增殖率。在体内，第21天时，GO-CTS组的伤口闭合率为85.5%±0.56%，FBS组为87.5%±1.75%，GO-CTS/FBS组为91.5%±1.03%，均显著高于对照组。此外，治疗组的新血管形成、上皮形成、胶原蛋白沉积和肉芽组织形成更为显著，在GO-CTS/FBS组中观察到了皮肤附属器。
含FBS的GO-CTS纳米片是一种有前途的皮肤组织工程生物材料，可有效启动和支持伤口愈合。
