Department of Plant Pathology, University of California, Davis, Davis, CA 95616.
Bayer Vegetables Research and Development, Woodland, CA 95695.
Plant Dis. 2022 Dec;106(12):3022-3026. doi: 10.1094/PDIS-04-22-0856-SC. Epub 2022 Nov 17.
Interest in industrial hemp () as a potential crop led to the establishment of commercial fields in a number of counties in California in 2019 and 2020. Plants in these fields developed different types of virus-like symptoms. The most prevalent type was stunted and bushy plants with distorted, upcurled, and yellowed leaves, which were similar to those associated with curly top disease (CTD) caused by the beet curly top virus (BCTV). This beet leafhopper-vectored virus is endemic in California and can cause economic losses to processing tomato production. Using a multiplex PCR test, BCTV infection was detected in 89% of hemp samples with CTD-like symptoms from Fresno, San Bernardino, and Ventura counties. Other symptom types had low incidence of BCTV infection and were associated with other factors. Hemp plants in California were infected only with the mild-type strains, BCTV-CO and BCTV-Wor, and often in mixed infection (43% of samples). Finally, using an infectious clone of a BCTV-CO isolate from hemp, we demonstrated that agroinoculated hemp plants developed these CTD-like symptoms, thereby fulfilling Koch's postulates for the disease.
人们对工业大麻（）作为一种潜在作物的兴趣，导致 2019 年和 2020 年加利福尼亚州的一些县建立了商业种植田。这些种植田中出现了不同类型的类似病毒的症状。最普遍的类型是植株矮小、枝叶茂密，叶片扭曲、上卷和发黄，这与甜菜曲顶病毒（BCTV）引起的曲顶病（CTD）相似。这种由叶蝉传播的病毒在加利福尼亚州流行，会给加工番茄生产造成经济损失。使用多重 PCR 检测，在弗雷斯诺、圣贝纳迪诺和文图拉县出现 CTD 样症状的 89%的大麻样本中检测到 BCTV 感染。其他症状类型的 BCTV 感染发生率较低，与其他因素有关。加利福尼亚州的大麻植物仅感染 BCTV-CO 和 BCTV-Wor 的温和型菌株，且通常为混合感染（占样本的 43%）。最后，使用来自大麻的 BCTV-CO 分离物的传染性克隆，我们证明了农杆菌接种的大麻植物会出现这些 CTD 样症状，从而满足了该疾病的科赫假设。
