Research Institute of Fast-Growing Trees/China Eucalypt Research Centre, Chinese Academy of Forestry, Zhanjiang 524022, Guangdong Province, China.
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0028, South Africa.
Plant Dis. 2022 Dec;106(12):3145-3153. doi: 10.1094/PDIS-01-22-0213-RE. Epub 2022 Nov 6.
The genus includes many aggressive plant-pathogenic species with a worldwide distribution. Calonectria leaf blight is one of the most prominent diseases of trees in Southeast Asian and South American plantations. Inoculation trials to evaluate pathogenicity of spp. typically use conidial suspensions but this is not possible for species that do not sporulate sufficiently in culture. is one of the species that is most aggressive to in China but most isolates fail to produce conidia in culture, requiring an alternative procedure for artificial inoculation. This study compared inoculations utilizing conidial and hyphal fragment suspensions. Two genotypes were used, and these were inoculated with different concentrations of hyphal fragments or conidia of three isolates. Three days after inoculation, the treated plants displayed similar disease symptoms, regardless of whether they had been inoculated with conidia or hyphal fragments. This was consistent for all isolates and also the different genotypes. The results demonstrate that hyphal fragment suspensions can be used to provide a reliable indication of isolate pathogenicity when conidia are not available for inoculation studies.
该属包含许多具有全球分布的侵袭性植物病原种。Calonectria 叶枯病是东南亚和南美种植园中最主要的 树病害之一。为了评估 种的致病性，通常使用分生孢子悬浮液进行接种试验，但对于在培养中不能充分产孢的物种来说，这是不可能的。是对中国 树最具侵袭性的物种之一，但大多数分离株在培养中无法产生分生孢子，因此需要一种替代的人工接种程序。本研究比较了利用分生孢子和菌丝片段悬浮液进行接种的效果。使用了两种 基因型，并将其分别用三种 分离株的不同浓度的菌丝片段或分生孢子进行接种。接种后 3 天，无论用分生孢子还是菌丝片段接种，处理后的 植株均表现出相似的病症。这对所有 分离株和不同的 基因型都是如此。结果表明，当无法获得接种研究用的分生孢子时，可以使用菌丝片段悬浮液来可靠地指示 分离株的致病性。
