Negi Nitika, Meena Rajendra K, Bhandari Maneesh S, Pandey Shailesh
Forest Pathology Discipline, Forest Protection Division, ICFRE-Forest Research Institute, Dehradun, India.
Genetics and Tree Improvement Division, ICFRE- Forest Research Institute, Dehradun, India.
Int Microbiol. 2024 Oct 7. doi: 10.1007/s10123-024-00602-x.
In this study, Calonectria eucalyptorum sp. nov. is described from the blighted leaves of Eucalyptus in India using morphological and multi-locus phylogenetic analyses. The new species belongs to the Calonectria cylindrospora species complex, and its unique microscopic features and DNA sequence information enable clear separation from the 12 currently accepted species in this complex. Conidia of the new taxon are slightly longer than those of its phylogenetic neighbors. Additionally, this species produces central as well as lateral stipe extensions, which is a feature not known for the other members of the C. cylindrospora species complex. Analyses of the combined partial calmodulin, histone, translation elongation factor-1α, and β-tubulin gene regions revealed a distinct phylogenetic position for C. eucalyptorum. Recombination analysis provided additional support for the new species hypothesis. Koch's postulates for the new taxon as a foliar pathogen of Eucalyptus were fulfilled. The discovery of novel and pathogenic Calonectria species is important because it sheds light on species diversity, potential threats, and disease control.
在本研究中，通过形态学和多位点系统发育分析，从印度桉树的枯萎叶片中描述了新物种桉木丽赤壳菌（Calonectria eucalyptorum sp. nov.）。该新物种属于圆柱丽赤壳菌物种复合体，其独特的微观特征和DNA序列信息使其能够与该复合体中目前已被接受的12个物种清晰区分开来。新分类群的分生孢子比其系统发育近缘种的分生孢子略长。此外，该物种会产生中央以及侧向的柄状延伸物，这是圆柱丽赤壳菌物种复合体其他成员所没有的特征。对钙调蛋白、组蛋白、翻译延伸因子-1α和β-微管蛋白基因区域的联合分析揭示了桉木丽赤壳菌独特的系统发育位置。重组分析为新物种假说提供了额外支持。该新分类群作为桉树叶部病原菌的柯赫氏法则得到了验证。发现新的致病丽赤壳菌物种很重要，因为这有助于了解物种多样性、潜在威胁和疾病控制。
